OmicsLink™ SNAP-Tag™ ORF Expression Clones |
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| More than 20,000 human full length ORFs are available for SNAP-Tag™ fusion in both T7 and CMV promoter driven expression systems. SNAP-Tag is a revolutionary protein tag which binds covalently and with high specificity to the tag's substrate, enabling proteins to be tagged with biotin or fluorphores, such as fluorescein and rhodamine, in vitro and in vivo. |
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The SNAP-Tag™ Technology |
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| SNAP-tag (gold) cleaves para-substituted benzyl guanines by transferring the substituted benzyl group to its active thiol and releasing free guanine. The resulting thio-ether bond covalently attaches a label(green) to the protein of interest (blue). Benzyl guanines are stable in biochemical conditions and no other protein reacts with this class of substance, thus the SNAP-tag reaction is highly specific. |
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Applications of SNAP-Tag Clones and Fusion Proteins: |
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Labeling of fusion proteins inside living cells:
Cell permeant biotin, fluorescein and rhodamine tag-substrates simplify the labeling of proteins in vivo.
A broad variety of experimental questions can be addressed, such as protein localization, protein dynamics and pulse-chase experiments.
Labeling of fusion proteins in extracts.
SNAP-tag allows specific labeling of fusion proteins in yeasts or other complex mixtures, without prior purification. |
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| Covalent immobilization of proteins to surfaces without prior purification. |
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ORF Expression Clones are also available in more than 80 other expression vector systems with different promoters and various N- or C-terminal tags: His, GST, MBP, eGFP, eYFP, AviTag™, HA, Flag, SUMO, and more.
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This product is manufactured under licensing agreement with Covalys Biosciences, AG. SNAP-Tag™ is a trademark of Covalys Biosciences, AG |
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