Introduction
RNAzol®RT is the most effective reagent for isolation of total RNA and small RNA from samples of various origins, including human, animal, plant, bacterial and viral origin.
RNAzol®RT separates RNA from other molecules in a single-step based on the interaction of phenol and guanidine with cellular components. No chloroform-induced phase separation is necessary to obtain pure RNA. It isolates mRNA and microRNA in separate fractions. Alternatively, total RNA containing all classes of RNA in a single fraction can be isolated. In addition, the patented reagent allows for the sequential isolation of RNA and DNA.
Advantages
Higher yield and quality
- Isolate pure and undegraded RNA that is ready for RT-PCR without DNase treatment
- Provides higher yield and quality of isolated RNA than previous reagents
- Isolate mRNA and microRNA in separate fractions
- Isolate total RNA in one fraction
- The isolation procedure can be completed in less than one hour
- No chloroform-induced phase separation is necessary
- Stable at room temperature for both storage and experimental procedure
References
- Chomczynski P, Reagents and methods for isolation of purified RNA, US Patent# 7,794,932, International Patents Pending.
- Chomczynski P, and Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol- chloroform extraction. Anal. Biochem. 162, 156 – 159.
- Chomczynski P (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532 – 537.
- Wilfinger W, Mackey K and Chomczynski P (1997) Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 22, 474 – 481.