All-in-One™ miRNA qRT-PCR Detection Kit and 700 validated miRNA primers — sensitive and practical quantitative profiling of miRNA expression that's economical

The All-in-One™ miRNA qRT-PCR Detection Kit combines PCR technology and SYBR® Green to make fast and accurate quantitative analysis of mature miRNAs from total RNA samples as small as 100 pg possible.

Providing an affordable two-step detection and quantification method of multiple miRNAs from one cDNA synthesis, the All-in-One is easy and efficient.

A novel optimized blend of poly A polymerase and reverse transcriptase with an RT buffer that was specifically developed for the
All-in-One Reverse Transcriptase Mix delivers maximum activity of both enzymes for robust and reproducible data.

When used with All-in-One validated miRNA primers the result is high-performance gene-expression profiling of all miRNAs.

All-in-One miRNA assays provides sensitivity and specificity you can afford

• Detect miRNA in 100 pg of total RNA
• Detect only mature miRNA
• Combine with GeneCopoeia validated miRNA-specific primers
• Design experiments with GeneCopoeia precursor miRNA plasmid constructs
• Follow-up with functional validation studies using GeneCopoeia ORF expression-ready clones

All-in-One miRNA assays are fast and easy

Figure 1. Overview of steps involved in the All-in-One miRNA qRT-PCR Detection Kit. During 3' polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.

All-in-One miRNA qPCR validated primers get the job done    

The All-in-One qPCR miRNA-specific primers are designed by a proprietary algorithm and validated for more than 700 human miRNAs.

When used in combination with the All-in-One SYBR® Green miRNA qRT-PCR kit, the All-in-One miRNA primers deliver reliable and reproducible high performance in quantitative PCR assays.

Primer validation

Figure 2A	Figure 2B

Figure 2. All-in-One miRNA qPCR pimers are validated to generate a single amplification of the correct size for the targeted miRNA and to yield a single dissociation curve peak. A cDNA pool, containing reverse transcribed products from 10 human tissue total RNA samples was used as the validation template. qPCR was performed using 0.2 uM primer with 2X All-in-One qPCR Mix. (2A) validated result for amplification curve (2B) validated result for melting curve.

Combine these tools with GeneCopoeia miRNA plasmid constructs and assays for comprehensive functional analysis studies.

All-in-One miR qRT-PCR Detection Kits — Order Now

Buy	Catalog#	Product	Description
	AOMD-Q020	All-in-One™ miRNA qRT-PCR Detection Kit* (20 RT and 200 qPCR reactions)	Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers
	AOMD-Q050	All-in-One™ miRNA qRT-PCR Detection Kit* (50 RT and 500 qPCR reactions)	Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers
	Variable	All-in-One miRNA qPCR Primer (20 µl x 500 reactions)	Validated miRNA human, mouse and rat primers

*miRNA-specific primers sold separately.    

Download All-in-One miRNA qRT-PCR Detection Kit product profile (PDF)

Download All-in-One miRNA qRT-PCR Detection Kit manual (PDF)

Download miRNA qPCR primer validation report sample (PDF)

Trademarks: SYBR® (Molecular Probes).