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miProfile™ miRNome miRNA qPCR Arrays

For high-throughput profiling of miRNA whole genome expression

 

Introduction

The miProfile human miRNome miRNA qPCR arrays are a set of nineteen 96-well plates covering 1,565 of best characterized and annotated miRNAs based on miRBase V17. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion. The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

To help best distinguish single-nucleotide mismatched miRNAs, 61 single-nucleotide mismatched miRNAs from the human miRNome are further grouped together on one 96-well plate. The miProfile human single-nucleotide mismatch miRNA qPCR array is available as stand-alone products for users who want to study these miRNAs using specific PCR conditions.

To view other miProfile miRNA qPCR arrays, click below:

 

Key Advantages

Validated miRNA primers

  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates

Largest coverage

  • More than 1,500 of best characterized and annotated miRNAs are covered based on miRBase V17

To Order

To Order

Important! Select your PCR instrument to find matching arrays:

If your PCR instrument is not listed here, please contact us at 1-866-360-9531 or inquiry@genecopoeia.com

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Select matching PCR array products:

 
Buy Cat. No. Product name Species Number of miRNAs Number of plates Document
QM001 miProfile™ human miRNome miRNA qPCR arrays Human 1,700 (miRBase V. 18) 21 x 96-well plates Datasheet
Array layout
Primer list
QM002 miProfile™ mouse miRNome miRNA qPCR arrays N/A 834 (miRBase V.18) 10 x 96-well plates Datasheet
Array layout
Primer list
QM003 miProfile™ human single-nucleotide mismatch miRNA qPCR arrays Human 61 1 x 96-well plates Datasheet
Array layout
Primer list
QM042 miProfile™ human miRNome miRNA qPCR arrays Human 1,700 (miRBase V.18) 5 x 384-well plates Datasheet
Array layout
Primer list
QM043 miProfile™ mouse miRNome miRNA qPCR arrays Mouse 834 (miRBase V.18) 3 x 384-well plates Datasheet
Array layout
Primer list


Select RT-PCR reagents

Buy Catalog# Product Description Price
QP020 RNAzol® RT RNA Isolation Reagent Easy isolation of mRNA, microRNA or total RNA $69
QP018 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (60 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $515
QP005 All-in-One™ qPCR Mix (4000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $1995
QP017 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (20 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $225
QP004 All-in-One™ qPCR Mix (1000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $635
QP002 All-in-One™ qPCR Mix (600 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $389
QP001 All-in-One™ qPCR Mix (200 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $169