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All-in-One™ First-Strand cDNA Synthesis Kit — robust cDNA from most any total or polyA RNA source

A robust experimental design delivers a universal kit suitable for first-strand cDNA synthesis from most any source of RNA

  • Efficient and easy
  • Reliable
  • Cost efficient

The All-in-One™ First-Strand cDNA Synthesis Kit includes a reverse transcriptase and a specialized set of reagents designed to yield cDNA that is optimal for gene cloning, cDNA library creation and quantitative PCR amplification.

The kit uses Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT (H–)) which is an RNA-dependent DNA polymerase that is used in cDNA synthesis with long RNA templates. The lack of RNase H activity is important in this application in that RNase H activity will start to degrade template during long incubation times which are required for producing long cDNAs. RNase H minus RT enables preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.

 

All-in-One First-Strand Synthesis Kit in action

An example showing assessment of reverse transcription efficiency using the All-in-One First Strand Synthesis Kit is shown below. The amplification results of different genes or gene regions using the oligo(dT) synthesized cDNA prepared from the All-in-One First-Strand cDNA Kit.

Figure 1. Synthesis Kit by oligo(dT) primer. The synthesized cDNA from human placenta was then used to amplify different gene regions by quantitative PCR using the All-in-One qPCR Mix (GeneCopoeia Catalog No. AOPR-0200). The positive amplification result of MACF1 indicates that up to 13 kb RNA sequence was reversed transcribed.

For universal qPCR reaction conditions using All-in-One™ qPCR Mix – click here!

 

Buy Catalog# Product Description Price
QP008 All-in-One™ First-Strand cDNA Synthesis Kit for gene qPCR array
(20 RT reactions)
M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $129
QP009 All-in-One™ First-Strand cDNA Synthesis Kit for gene qPCR array
(60 synthesis reactions)
M-MLV RT (RNase H-), Reaction Buffer, RNase Inhibitor, dNTPs, Oligo(dT)18, Random Primer, dd H2O and spike-in control (for use with gene qPCR arrays) $285
  Variable All-in-One™ qPCR Primer
(200 reactions)
Validated gene-specific for human, mouse and rat toensure specificity and sensitivity

 

 

 

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