Recombinant Human TYMS Protein Data Sheet

Catalog #	hRP-T0406-EF012
Size	100 μg
Protein Name	Human thymidylate synthetase
Protein Symbol	TYMS
Original Source	Homo sapiens
Expression System	E.coli
GenBank Accession #	NM_001071.1
Uniprot Accession #	P04818
Description	Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase.
Application	WB, ELISA, IP, antibody production, protein array
Fusion tag	N-His
Peptide Length	328aa(including fusion tag)
Molecular Weight	37.7kDa(including fusion tag)
pI	7.2
Activity	NA
Storage	Storage buffer: 20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0. Store at -80°C and avoid repeated freeze-thaw cycles.
Purity

Reference:

• Thymidylate synthase pharmacogenetics
• G>C SNP of thymidylate synthase with respect to colorectal cancer
• Thymidylate synthase structure, function and implication in drug discovery
• Thymidylate synthase as a prognostic biomarker for locally advanced rectal cancer after multimodal treatment
• Human thymidylate synthase with loop 181-197 stabilized in an inactive conformation: ligand interactions, phosphorylation, and inhibition profiles
• Crystal structures of thymidylate synthase mutant R166Q: structural basis for the nearly complete loss of catalytic activity