Protein gel staining and Western Bloting are important tools in proteomics (Figure 1). GeneCopoeia offers two distinct protein gel stains: 1) Coomassie® Blue Fast Stain, a ready-to-use product that does not require methanol or acetic acid fixatives or destains; 2) eLuminol™ Protein Gel Stain, a highly-sensitive fluorescent stain designed to detect proteins in SDS polyacrylamide gels. In addition, GeneCopoeia also offers a phosphoprotein selective stain: Phospho-Tag™ Phosphoprotein Gel Stain. GeneCopoeia’ ECL Western Blotting Detection Kit provides highly sensitive detection on immunoblots. Besides, GeneCopoeia also offers two protein assay kits: 1) Bradford Protein Assay Kit; and 2) BCA Protein Assay Kit.
Figure 1. Protein gel analysis workflow
Protein Gel Stain Selection Guide
|Coomassie® Blue Fast Stain||eLuminol™ Protein Gel Stain||Phospho-Tag™ Phosphoprotein Gel Stain|
|Sensitivity||20 ng||0.5 ng||1 ng|
|Ease of use||Ready to use||Supplied as stock solution||Ready to use|
|Time required||2 hr (standard)
20 min (microwave)
|90 min (standard)
30 min (microwave)
|4 hr (standard)
2 hr (microwave)
|MS spec. compatible||Yes||Yes||Yes|
Figure 2. Gel stain results with Coomassie® Blue Fast Stain (left), eLuminol™ Protein Gel Stain (middle), and Phospho-Tag™ Phosphoprotein Gel Stain, followed by eLuminol™ total protein stain (right). Two-fold serial dilutions (from left to right) of protein marker were separated via SDS-PAGE and then stained with above stains.
|ECL Western Blotting Detection Kit|
Figure 3. Detection of protein in Western Blots with ABP kit (left) and Millipore Kit (right).
|Bradford Protein Assay Kit||BCA Protein Assay Kit|
Figure 4. BSA standard curve with Bradford Protein Assay Kit (left) and BCA Protein Assay Kit (right).