Introduction
Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of over 20,000 human and 18,000 mouse promoters using live cell assays.
Each transfection-ready promoter clone contains a 1.2-1.5 kb insert, corresponding to the 5'-flanking promoter sequence located approximately 1.5 kb upstream and up to 200 bp downstream of the transcription start site (TSS) of a specific human gene. This insert is placed upstream of the GLuc reporter gene. Since the putative cis-acting enhancer elements are expected to exist in the cloned promoter region, the promoter luciferase activity observed during the reporter assay closely resembles the actual promoter regulation of these genes within human cells.
GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization.
Figure 1. How GLuc-ON promoter clones work.
Advantages |
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Live cell assays
- Naturally secreted GLuc reporter
- No lysis of the cells is necessary
- Save samples, reduce variations, and simplify experiments for applications such as pulse chase analysis,etc.
Real-time study
- Data is generated quickly
- Closely resembles real-time activities
Dual secreted reporter system
- Secreted GLuc and SEAP
- Enables transfection-normalization for accurate across-sample comparison
High-throughput compatible
- Group or pathway study compatible
- High sample number compatible
High sensitivity
- GLuc is 1000-fold more sensitive than firefly or Renilla luciferase
Convenience
- All promoter clones are transfection-ready
Gaussia luciferase
GLuc-ON promoter clones use a modified GLuc (mGLuc) as the reporter gene, which generates a highly stable signal and overcomes the quick signal decay commonly observed with humanized wild type GLuc (wtGLuc).
Figure 2. Signal stability of mGLuc (blue) and wtGLuc (red). Left: assay buffer with a stabilizer; Right: regular assay buffer (Secrete-Pair™ dual luminescence assay kit)
Dual-reporter system
Dual-reporter vectors are available for the GLuc-ON promoter clones. The secondary reporter, secreted Alkaline Phosphatase (SEAP), serves as an internal control. The dual-reporter system enables transfection normalization for accurate cross-sample comparison.
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Figure 3. Normalized promoter activities in H1B1B and HEK293T cells. Dual-reporter promoter clones or controls were transfected into two cell lines in duplicates. Samples were analyzed 24 hrs (HEK293T) and 48 hrs (H1B1B) after transfection. NEG (containing non-promoter sequence) and EMPTY (no promoter in the vector) are negative controls. |
Vectors and Controls
Vector choices |
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| Vector | Reporter gene | Tracking gene | Selection marker | Vector type |
| pEZX-PG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | Non-viral |
| pEZX-PG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | Non-viral |
| pEZX-PF02 | eGFP | N/A* | Puromycin | Non-viral |
| pEZX-PM02 | mCherry | N/A* | Puromycin | Non-viral |
| pEZX-LvPG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | HIV Lentiviral |
| pEZX-LvPG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPF02 | eGFP | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPM02 | mCherry | N/A* | Puromycin | HIV Lentiviral |
| pEZX-LvPM03 | tdTomato | N/A* | Puromycin | HIV Lentiviral |
* A separate vector is available for SEAP expression.
** These Gaussia luciferase cloning vectors are available for purchase.
Control clones and SEAP expression clone
Tear Away License
To Order
Pre-designed promoter clonesSearch over 20,000 human and 18,000 mouse pre-designed promoter reporter clones in your choice of vector.
Promoter lentivirusGeneCopoeia offers lentivirus as a custom service.
Custom promoter clone services
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Want to compare various length or regions of your promoter? You can custom-order them.
Please complete our Custom Promoter Clone Inquiry and Quotation form,
email inquiry@genecopoeia.com or call: 866-360-9531 or 301-762-0888
| Download warranty |
| GLuc-ON™ Promoter Reporter Clones Cancellation, QA, License, and Warranty |
Related Products
Related products
- Secrete-Pair™ Dual Luminescence Assay Kit
- Genome-TALER™ TALE-TF Custom Services
- TF-Detect™ Human P53 Activity Assay Kit
- TF-Detect™ AP-1/c-Jun Activity Assay Kit
- MethylAffinity™ Methylated DNA Enrichment Kit
Publications
Publications using GeneCopoeia Promoter-Reporter Clones
2017
- Druzd, D., et al. (2017). Lymphocyte Circadian Clocks Control Lymph Node Trafficking and Adaptive Immune Responses. Immunity doi: 10.1016/j.immuni.2016.12.011 [GLuc-ON™ S1pr1 promoter-reporter clone]
- Alvarado, A.G., et al. (2017). Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression. Cell Stem Cell doi: 10.1016/j.stem.2016.12.001 [GLuc-ON™ SOX2, OCT4, and NANOG promoter-reporter clones]
- Tang, Q., et al. (2017). Emodin Increases Expression of Insulin-Like Growth Factor Binding Protein 1 through Activation of MEK/ERK/AMPKα and Interaction of PPARγ and Sp1 in Lung Cancer. Cellular Physiology and Biochemistry doi: 10.1159/000456281. [GLuc-ON™ IGFBP1 promoter-reporter clone]
- Wang, X., et al. (2017). Macrophages induce AKT/β-catenin-dependent Lgr5+ stem cell activation and hair follicle regeneration through TNF. Nature Communications doi: 10.1038/ncomms14091 [GLuc-ON™ LGR5 promoter-reporter clone]
- Feng, W., et al. (2017). SOX10 induced Nestin expression regulates cancer stem cell properties of TNBC cells. Biochemical and Biophysical Research Communications doi: 10.1016/j.bbrc.2017.02.014 [NES promoter-reporter clone]
- Wu, D.W., et al. (2017). The YAP1/SIX2 axis is required for DDX3 -mediated tumor aggressiveness and cetuximab resistance in KRAS -wild -type colorectal cancer. Theranostics doi: 10.7150/ thno .18175 [GLuc-ON™ YAP1 and SIX2 promoter-reporter clones]
- Lim, J.P., et al. (2017). YBX1 gene silencing inhibits migratory and invasive potential via CORO1C in breast cancer in vitro. BMC Cancer doi: 10.1186/s12885-017-3187-7 [GLuc-ON™ CORO1C promoter-reporter clone]
- Cho, Y.S., et al. (2017). COMPOSITIONS COMPRISING A MITOFUSIN INHIBITOR FOR PROMOTING CELL REPROGRAMMING AND A USE THEREOF United States Patent Application 20170051254 [GLuc-ON™ Mfn1 promoter-reporter clone]
- Sun, J., et al. (2017). HMGA2 regulates CD44 expression to promote gastric cancer cell motility and sphere formation. Am J Cancer Res 2017;7(2) :260-274 [GLuc-ON™ c-Myc promoter-reporter clone]
- Mondello, P., et al. (2017). Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265 mutations. JC Insight doi: doi.org/10.1172 [GLuc-ON™ MyD88 promoter-reporter clone]
- Shen, Z.H., et al. (2017). HOXA10 promotes nasopharyngeal carcinoma cell proliferation and invasion via inducing the expression of ZIC2. European Review for Medical and Pharmacological Sciences 2017; 21: 945-952 [GLuc-ON™ ZIC2 promoter-reporter clone]
- Shen, C.J., et al. (2017). MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer. PLoS ONE doi: 10.1371/journal.pone.0174487 [GLuc-ON™ MMP1 promoter-reporter clone]
- Wu, J.J., et al. (2017). Interplay of DNA methyltransferase 1 and EZH2 through inactivation of Stat3 contributes to β-elemene-inhibited growth of nasopharyngeal carcinoma cells. Scientific eports doi: 10.1038/s41598-017-00626-6 [GLuc-ON™ EZH2 promoter-reporter clone]
- Qu, L.P., et al. (2017). CDH17 is a downstream effector of HOXA13 in modulating the Wnt/ β -catenin signaling pathway in gastric cancer. European Review for Medical and Pharmacological Sciences 2017; 21: 1234-1241 [GLuc-ON™ CDH17 promoter-reporter clone]
- Morel, A. P., et al. (2017). A stemness-related ZEB1–MSRB3 axis governs cellular pliancy and breast cancer genome stability. Nature Medicine doi: 10.1038/nm.4323 [GLuc-ON™ MSRB3 promoter-reporter clone]
- Liu, J., et al. (2017). DDA1 is induced by NR2F6 in ovarian cancer and predicts poor survival outcome. European Review for Medical and Pharmacological Sciences 2017; 21: 1206 -1213 [GLuc-ON™ DDA1 promoter-reporter clone]
- Wang, L., et al. (2017). FSCN1 is upregulated by SNAI2 and promotes epithelial to mesenchymal transition in head and necksquamous cell carcinoma. Cell Biology International doi: 10.1002/cbin.10786 [GLuc-ON™ FSCN1 promoter-reporter clone]
- Larson, M.E., et al. (2017). Selective lowering of synapsins induced by oligomeric α-synuclein exacerbates memory deficits. Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.1704698114 [GLuc-ON™ SYN2 promoter-reporter clone]
- Azeem, W., wt al. (2017). An androgen response element driven reporter assay for the detection of androgen receptor activity in prostate cells. PLoS ONE doi: 10.1371/journal.pone.0177861 [ARE promoter-reporter clone]
- Li, D., et al. (2017). Hepatic hypoxia-inducible factors inhibit PPARα expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity. Free Radical Biology and Medicine doi: 10.1016/j.freeradbiomed.2017.06.002 [GLuc-ON™ PPARα promoter-reporter clone]
- TianJ., et al. (2017). E2F8 confers cisplatn resistance to ER+ breast cancer cells via transcriptionally activating MASTL. Biomedicine & Pharmacotherapy doi: 10.1016/j.biopha.2017.05.118 [MASTL promoter-reporter clone]
2016
- Liao, BM. et al. (2016) Flavin-Containing Monooxygenase 3 Reduces Endoplasmic Reticulum Stress in Lipid-Treated Hepatocytes. Molecular Endocrinology,DOI: http://dx.doi.org/10.1210/me.2015-1217. [FMO3 promoter clone and secreted pair luciferase kit]
- Dougherty, E.J., et al. (2016). Mineralocorticoid Receptor (MR) Trans-Activation of Inflammatory AP-1 Signaling: Dependence on DNA Sequence, MR Conformation, and AP-1 Family Member Expression. The Journal of Biological Chemistry doi: 10.1074/jbc.M116.732248 [human XIRP1 promoter clone]
- Li, L., et al. (2016). Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I. Journal of Experimental & Clinical Cancer Research doi: 10.1186/s13046-016-0388-x [EZH2 promoter-reporter clone]
- Mao, C., et al. (2016). Double-edged-sword effect of IL-1β on the osteogenesis of periodontal ligament stem cells via crosstalk between the NF-κB, MAPK and BMP/Smad signaling pathways. Cell Death and Disease doi: 10.1038/cddis.2016.204 [BMP2 promoter-reporter clone]
- Gujar, R., et al. (2016). c-Src Suppresses Dendritic Cell Antitumor Activity via T Cell Ig and Mucin Protein-3 Receptor. The Journal of Immunology doi: 10.4049/jimmunol.1600104 [mouse wild tyoe and mutant HAVCR2 promoter-reporter clones]
- Chen, J. et al. (2016). Vitamin D Deficiency Promotes Liver Tumor Growth in Transforming Growth Factor-β/Smad3-Deficient Mice Through Wnt and Toll-like Receptor 7 Pathway Modulation. Scientific Reports doi: 10.1038/srep30217 [TLR7 Promoter-reporter clone]
- Nakajima, S., et al. (2016). Fungal-derived neoechinulin B as a novel antagonist of liver X receptor, identified by chemical genetics using hepatitis C virus cell culture system . Journal of Virology doi: 10.1128/JVI.00856-16 [SCD-1 promoter reporter clone]
- Zhu, L., et al. (2016). Knockdown of Sall4 inhibits intrahepatic cholangiocarcinoma cell migration and invasion in ICC-9810 cells. OncoTargets and Therapy doi: 10.2147/OTT.S107214 [PTEN and Bmi-1 promoter clones]
- Rehman, M., et al. (2016). PlexinD1 Is a Novel Transcriptional Target and Effector of Notch Signaling in Cancer Cells. PLoS ONE doi: 10.1371/journal.pone.0164660 [Hes1, PlexinD1promoter clones]
- Trueba-Saiz, A., et al. (2016). Circulating Insulin-like Growth Factor I Regulates Its Receptor in the Brain of Male Mice. Endocrinology doi: 10.1210/en.2016-1468 [IGF-IR promoter-Gaussia luciferase reporter lentiviral clone]
- Ashouri, A., et al. (2016). Pan-cancer transcriptomic analysis associates long non-coding RNAs with key mutational driver events. Nature Communications doi: 10.1038/ncomms13197 [Gaussia luciferase-Secreted alkaline phosphatase cloning vector pEZX-PG04]
- Chu, Q., et al. (2016). Extracellular serglycin upregulates the CD44 receptor in an autocrine manner to maintain self-renewal in nasopharyngeal carcinoma cells by reciprocally activating the MAPK/β-catenin axis. Cell Death and Disease doi: 10.1038/cddis.2016.287 [GLuc-ON™ CD44 promoter-reporter clone]
- Xiang, S., et al. (2016). Activation of AMPKα mediates additive effects of solamargine and metformin on suppressing MUC1 expression in castration-resistant prostate cancer cells. Scientific Reports doi:10.1038/srep36721 [GLuc-ON™ AMPKα ORF expression clone]
- Xiang, S., et al. (2016). Activation of AMPKα mediates additive effects of solamargine and metformin on suppressing MUC1 expression in castration-resistant prostate cancer cells. Scientific Reports doi:10.1038/srep36721 [GLuc-ON™ MUC1 promoter-reporter clone]
- Holler, et al. (2016). Trehalose upregulates progranulin expression in human and mouse models of GRN haploinsufficiency: a novel therapeutic lead to treat frontotemporal dementia. Molecular Neurodegeneration doi: 10.1186/s13024-016-0114-3 [GLuc-ON™ PGRN promoter-reporter clone]
- Revandkar, A., et al. (2016). Inhibition of Notch pathway arrests PTEN-deficient advanced prostate cancer by triggering p27-driven cellular senescence. Nature Communications doi: 10.1038/ncomms13719 [GLuc-ON™ ADAM7 promoter clone]
- Xie, X., et al. (2016). c-Jun N-terminal kinase promotes stem cell phenotype in triple-negative breast cancer through upregulation of Notch1 via activation of c-Jun. Oncogene doi: 10.1038/onc.2016.417 [GLuc-ON™ Notch1 promoter-reporter clone]
2015
- Natunen, T. et al. (2015) Relationship between ubiquilin-1 and BACE1 in human Alzheimer's disease and APdE9 transgenic mouse brain and cell-based models. Neurobiology of disease,DOI: 10.1016/j.nbd.2015.11.005. [human BACE1 containing endogenous 5′UTR, BACE1-Myc (human BACE1 with a CMV promoter and a 3′-myc-tag sequence producing BACE1 protein containing a myc tag in the C-terminus)]
- C Authors, R Trivedi . et al. (2015) JBC Papers in Press. Published on December 9, 2015 as Manuscript M115. 687152. JBC,http://www.jbc.org/cgi/doi/10.1074/jbc.M115.687152. [Runx2 promoter clones (Cat# MPRM13038)]
- Muralidharan, R. et al. (2015) HuR-targeted nanotherapy in combination with AMD3100 suppresses CXCR4 expression, cell growth, migration and invasion in lung cancer. Cancer Gene Therapy,ancer Gene Therapy (2015) 22, 581–590; doi:10.1038/cgt.2015.55. [Promotor reporter clone for CXCR4]
- Maeng, YS. et al. (2015) TGFBIp regulates differentiation of EPC (CD133+ C‐kit+ Lin‐cells) to EC through activation of the Notch signaling pathway. STEM CELLS,DOI: 10.1002/stem.2003. [pEZX-DLL1 pro- moter luciferase plasmids (-1.2 Kbp)]
- Li, C. et al. (2015) Noncanonical STAT3 Activation Regulates Excess TGF-β1 and Collagen I Expression in Muscle of Stricturing Crohn's Disease. The Journal of Immunology,doi: 10.4049/jimmunol.1401779. [promoter reporter clone for human TGFB1 (HPRM13178-PG04), EndoFectin Lenti transfection reagent (EFP1003)]
- Zhang , Z. et al. (2015) BET Bromodomain Inhibition as a Therapeutic Strategy in Ovarian Cancer by Downregulating FoxM1. Theranostics,doi:10.7150/thno.13178. [human FoxM1 promoter clone]
2014
- Ryu , JM. et al. (2014) Autotaxin‐LPA Axis regulates hMSC migration by adherent junction disruption and cytoskeletal rearrangement via LPAR1/3‐dependent PKC/GSK3β/β‐catenin and PKC/Rho GTPase Pathways . STEM CELLS,DOI: 10.1002/stem.1882. [CDH-1 promoter construct]
- Zhang, H. et al. (2014) The critical role of myeloid-derived suppressor cells and FXR activation in immune-mediated liver injury. Journal of Autoimmunity, doi: 10.1016/j.jaut.2014.02.010. [MPRM11909-PG04]
- Rubie, C. et al. (2014) Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells. Journal of translational medicine, 2014 Feb 21;12:48. doi: 10.1186/1479-5876-12-48.. [Luc CCR6 vector, Luc No CCR6 vector]
2013
- Klimosch, SN. et al (2013) Functional TLR5 genetic variants affect human colorectal cancer survival. Cancer Research, 73(24); 7232–42.. [Promoter Reporter ( MYD88 and ACAA1)].
- Shen, Q. et al (2013) Adipocyte reporter assays: Application for identification of anti‐inflammatory and antioxidant properties of mangosteen xanthones. Molecular Nutrition & Food Research, DOI: 10.1002/mnfr.201300181. [Human Fabp4 promoter vector with eCFP reporter].
- Celardo, I. et al. (2013) Caspase-1 is a novel target of p63 in tumor suppression. Cell Death and Disease (2013) 4, e645; doi:10.1038/cddis.2013.175. [caspase-1 promoter plasmid in pEZX-PG02 vector].
- Zheng, H. et al. (2013) Glycogen synthase kinase-3 beta regulates Snail and β-catenin expression during Fas-induced epithelial–mesenchymal transition in gastrointestinal cancer. European Journal of Cancer, Available online 9 April 2013. [CDH1 promoter construct].
- Mills, L.D. et al. (2013) Loss of the Transcription Factor GLI1 Identifies a Signaling Network in the Tumor Microenvironment Mediating KRAS-Induced. Journal of Biological Chemistry, March 12, 2013, doi: 10.1074/jbc.M112.438846. [mouse SHH promoter clone].
- Schank, JR. et al (2013) Tacr1 Gene Variation and Neurokinin 1 Receptor Expression Is Associated with Antagonist Efficacy in Genetically Selected Alcohol-Preferring Rats. Biological Psychiatry Journal, PII: S0006-3223(13)00043-7, doi:10.1016/j.biopsych.2012.12.027. [custom Tacr1 promoter luciferase construct].
2012
- Petrella, B. et al. (2012) Interleukin-1 beta and transforming growth factor-beta 3 cooperate to activate matrix metalloproteinase expression and invasiveness in A549 lung adenocarcinoma … Cancer Letters, Volume 325, Issue 2, 28 December 2012, Pages 220–226. [MMP10 promoter clone].