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GLuc-ON™ Promoter Reporter Clones

Introduction

Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of over 20,000 human and 18,000 mouse promoters using live cell assays.

Each transfection-ready promoter clone contains a 1.2-1.5 kb insert, corresponding to the 5'-flanking promoter sequence located approximately 1.5 kb upstream and up to 200 bp downstream of the transcription start site (TSS) of a specific human gene. This insert is placed upstream of the GLuc reporter gene. Since the putative cis-acting enhancer elements are expected to exist in the cloned promoter region, the promoter luciferase activity observed during the reporter assay closely resembles the actual promoter regulation of these genes within human cells.

GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization.

Promoter reporter clones mechanism of action

Figure 1. How GLuc-ON promoter clones work.

 

Advantages

Live cell assays

  • Naturally secreted GLuc reporter
  • No lysis of the cells is necessary
  • Save samples, reduce variations, and simplify experiments for applications such as pulse chase analysis,etc.

Real-time study

  • Data is generated quickly
  • Closely resembles real-time activities

Dual secreted reporter system

  • Secreted GLuc and SEAP
  • Enables transfection-normalization for accurate across-sample comparison

High-throughput compatible

  • Group or pathway study compatible
  • High sample number compatible

High sensitivity

  • GLuc is 1000-fold more sensitive than firefly or Renilla luciferase

Convenience

  • All promoter clones are transfection-ready

 

Gaussia luciferase

GLuc-ON promoter clones use a modified GLuc (mGLuc) as the reporter gene, which generates a highly stable signal and overcomes the quick signal decay commonly observed with humanized wild type GLuc (wtGLuc).

Signal stability of mGluc and wtGlucSignal stability of mGluc and wtGluc

Figure 2. Signal stability of mGLuc (blue) and wtGLuc (red). Left: assay buffer with a stabilizer; Right: regular assay buffer (Secrete-Pair™ dual luminescence assay kit)

 

Dual-reporter system

Dual-reporter vectors are available for the GLuc-ON promoter clones. The secondary reporter, secreted Alkaline Phosphatase (SEAP), serves as an internal control. The dual-reporter system enables transfection normalization for accurate cross-sample comparison.

 

Figure 3. Normalized promoter activities in H1B1B and HEK293T cells. Dual-reporter promoter clones or controls were transfected into two cell lines in duplicates. Samples were analyzed 24 hrs (HEK293T) and 48 hrs (H1B1B) after transfection. NEG (containing non-promoter sequence) and EMPTY (no promoter in the vector) are negative controls.

Vectors and Controls

Vector choices

Vector Reporter gene Tracking gene Selection marker Vector type
pEZX-PG04 Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin Non-viral
pEZX-PG02 Gaussia luciferase (GLuc) N/A* Puromycin Non-viral
pEZX-PF02 eGFP N/A* Puromycin Non-viral
pEZX-PM02 mCherry N/A* Puromycin Non-viral
pEZX-LvPG04 Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin HIV Lentiviral
pEZX-LvPG02 Gaussia luciferase (GLuc) N/A* Puromycin HIV Lentiviral
pEZX-LvPF02 eGFP N/A* Puromycin HIV Lentiviral
pEZX-LvPM02 mCherry N/A* Puromycin HIV Lentiviral

* A separate vector is available for SEAP expression.

 

Control clones and SEAP expression clone

Buy Catalog# Product Description Vector Price
GAPDH-PF02 GAPDH positive control clone House keeping gene promoter positive control in single GFP reporter vector pEZX-PF02 $375
GAPDH-LvPF02 GAPDH positive control clone House keeping gene promoter positive control in single GFP reporter lentiviral vector pEZX-LvPF02 $595
GAPDH-PG02 GAPDH positive control clone House keeping gene promoter positive control in single-reporter vector pEZX-PG02 $375
GAPDH-LvPG02 GAPDH positive control clone House keeping gene promoter positive control in single-reporter lentiviral vector pEZX-LvPG02 $595
GAPDH-PG04 GAPDH positive control clone House keeping gene promoter positive control in dual-reporter vector pEZX-PG04 $495
GAPDH-LvPG04 GAPDH positive control clone House keeping gene promoter positive control in dual-reporter lentiviral vector pEZX-LvPG04 $745
GAPDH-PM02 GAPDH positive control clone House keeping gene promoter positive control in single mCherry reporter vector pEZX-PM02 $375
GAPDH-LvPM02 GAPDH positive control clone House keeping gene promoter positive control in single mCherry reporter lentiviral vector pEZX-LvPM02 $595
NEG-PF02 Negative control clone Non-promoter sequence negative control in single GFP reporter vector pEZX-PF02 $375
NEG-LvPF02 Negative control clone Non-promoter sequence negative control in single GFP reporter lentiviral vector pEZX-LvPF02 $595
NEG-PG02 Negative control clone Non-promoter sequence negative control in single-reporter vector pEZX-PG02 $375
NEG-LvPG02 Negative control clone Non-promoter sequence negative control in single-reporter lentiviral vector pEZX-LvPG02 $595
NEG-PG04 Negative control clone Non-promoter sequence negative control in dual-reporter vector pEZX-PG04 $495
NEG-LvPG04 Negative control clone Non-promoter sequence negative control in dual-reporter lentiviral vector pEZX-LvPG04 $745
NEG-PM02 Negative control clone Non-promoter sequence negative control in single mCherry reporter vector pEZX-PM02 $375
NEG-LvPM02 Negative control clone Non-promoter sequence negative control in single mCherry reporter lentiviral vector pEZX-LvPM02 $595
SEAP-PA01 Secreted alkaline phosphatase (SEAP)
Expression clone
Express SEAP.
Can be used for co-transfection with promoter clone
pEZX-PA01 $395
SEAP-LvPA02 Secreted alkaline phosphatase (SEAP) expression clone Express SEAP in a lentiviral vector. Can be used for co-transduction with a promoter clone pEZX-PA02 $595

 

Tear Away License

Tear away license for gaussia luciferase users

To Order

Pre-designed promoter clones

Search over 20,000 human and 18,000 mouse pre-designed promoter reporter clones in your choice of vector.

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Promoter lentivirus

GeneCopoeia offers lentivirus as a custom service. Request a quote for lentivirus production services.

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Custom promoter clone services

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Want to compare various length or regions of your promoter? You can custom-order them.

Please complete our Custom Promoter Clone Inquiry and Quotation form,
email inquiry@genecopoeia.com or call: 866-360-9531 or 301-762-0888

 

Download warranty
 GLuc-ON™ Promoter Reporter Clones Cancellation, QA, License, and Warranty

Publications

Promoter reporter clone publications

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