GLuc-ON™ Promoter Reporter Clones

Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed to detect the real-time activities of over 20,000 human promoters using live cell assays.

Each transfection-ready promoter clone contains a 1.2-1.5 kb insert, corresponding to the 5'-flanking sequence located approximately 1.5 kb upstream and up to 200 bp downstream of the transcription start site (TSS) of a specific human gene. This insert is placed upstream of the GLuc reporter gene. Since the putative cis-acting enhancer elements are expected to exist in the cloned promoter region, the luciferase activity observed during the reporter assay closely resembles the actual promoter regulation of these genes within human cells.

GLuc-ON promoter clones can be ordered as pre-designed or custom-built in one of our robust vector systems. Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses either GLuc or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization.

Figure 1. How GLuc-ON promoter clones work.

Live cell assays
• Naturally secreted GLuc reporter
• No lysis of the cells is necessary
• Save samples, reduce variations, and simplify experiments for applications such as pulse chase analysis,etc.

Real-time study
• Data is generated quickly
• Closely resembles real-time activities

Dual secreted reporter system
• Secreted GLuc and SEAP
• Enables transfection-normalization for accurate across-sample comparison

High-throughput compatible
• Group or pathway study compatible
• High sample number compatible

High sensitivity
• GLuc is 1000-fold more sensitive than firefly or Renilla luciferase

Convenience
• All promoter clones are transfection-ready

Vector choices