Figure 1. How GLuc-ON promoter clones work.
Advantages |
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Live cell assays
- Naturally secreted GLuc reporter
- No lysis of the cells is necessary
- Save samples, reduce variations, and simplify experiments for applications such as pulse chase analysis,etc.
Real-time study
- Data is generated quickly
- Closely resembles real-time activities
Dual secreted reporter system
- Secreted GLuc and SEAP
- Enables transfection-normalization for accurate across-sample comparison
High-throughput compatible
- Group or pathway study compatible
- High sample number compatible
High sensitivity
- GLuc is 1000-fold more sensitive than firefly or Renilla luciferase
Convenience
- All promoter clones are transfection-ready
Gaussia luciferase
GLuc-ON promoter clones use a modified GLuc (mGLuc) as the reporter gene, which generates a highly stable signal and overcomes the quick signal decay commonly observed with humanized wild type GLuc (wtGLuc).
Figure 2. Signal stability of mGLuc (blue) and wtGLuc (red). Left: assay buffer with a stabilizer; Right: regular assay buffer (Secrete-Pair™ dual luminescence assay kit)
Dual-reporter system
Dual-reporter vectors are available for the GLuc-ON promoter clones. The secondary reporter, secreted Alkaline Phosphatase (SEAP), serves as an internal control. The dual-reporter system enables transfection normalization for accurate cross-sample comparison.
Vector choices |
Vector | Reporter gene | Tracking gene | Selection marker | Vector type |
pEZX-PG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | Non-viral |
pEZX-PG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | Non-viral |
pEZX-PF02 | eGFP | N/A* | Puromycin | Non-viral |
pEZX-PM02 | mCherry | N/A* | Puromycin | Non-viral |
pEZX-LvPG04** | Gaussia luciferase (GLuc) | Secreted alkaline phosphatase (SEAP) | Puromycin | HIV Lentiviral |
pEZX-LvPG02** | Gaussia luciferase (GLuc) | N/A* | Puromycin | HIV Lentiviral |
pEZX-LvPF02 | eGFP | N/A* | Puromycin | HIV Lentiviral |
pEZX-LvPM02 | mCherry | N/A* | Puromycin | HIV Lentiviral |
pEZX-LvPM03 | tdTomato | N/A* | Puromycin | HIV Lentiviral |
* A separate vector is available for SEAP expression. ** These Gaussia luciferase cloning vectors are available for purchase.
Control clones and SEAP expression clone
Tear Away License
Want to compare various length or regions of your promoter? You can custom-order them. Please complete our Custom Promoter Clone Inquiry and Quotation form, email inquiry@genecopoeia.com or call: 866-360-9531 or 301-762-0888
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GLuc-ON™ Promoter Reporter Clones Cancellation, QA, License, and Warranty |