GeneCopoeia’s GLuc-ON™ PR transcriptional response element (TRE) clone enables analysis of the activity of signal transduction mediated by progesterone receptors (PR). Progesterone receptors are transcription factors that, in response to stimulation by progesterone, lead to proliferation of breast tissue. Aberrant function and/or expression of PRs is an important factor in development of breast cancer
The GLuc-ON™ PR TRE clone contains tandem repeats of PR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The PR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ PR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ PR TRE by exogenous expression of PR. 293T cells were were co-transfected in the presence of 10 nM progesterone with (left to right) negative TRE clone + negative expression clone, negative TRE clone + PR expression clone, PR TRE clone + negative expression clone, or PR TRE clone + PR expression clone. Transfected cells were incubated for 40 hours. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.