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OmicsLink™ Expression-Ready ORF cDNA Clones

Product Information

OmicsLink™ Expression-Ready ORF Collection

GeneCopoeia offers the largest collection of ORF clones, with genome-wide coverage for human and mouse. All ORFs can be readily expressed in combination with a variety of fusion tags (fluorescence, antibody, solubility, purification or no tag), promoters and selection markers, for easy expression in different host systems.

Nextday-ORF™ Collection

Our Nextday-ORF™ collection is a subset of our OmicsLink™ clones, with guaranteed next-day shipping for 140,000 premade ORF clones in mammalian and lentiviral expression systems, as well as the Gateway® shuttle vector.

Secondary Clone Discount 

When you order more than one clone for the same ORF (with the same gene accession #) in different vector systems, you can purchase each additional clone at a discount price of $200.

 

 

 Key advantages

  • Next-day shipping of 140,000 ORF clones in mammalian, lentiviral, and Gateway® PLUS systems
  • Largest vector collection in the industry, with 200+ vector types (click here)
  • All premade ORF clones available in clone sets by gene groups 
  • All ORF clones are fully sequence-verified and guaranteed

 

OmicsLink™ ORF Clone Expression Systems

Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-M01 CMV Mammalian Neomycin N-His N/A
pReceiver-M51 CMV Mammalian Neomycin C-His+IRES-eGFP N/A
pReceiver-M77 CMV Mammalian Neomycin C-His N/A
pReceiver-M02 CMV Mammalian Neomycin Untagged N/A
pReceiver-M67 CMV Mammalian Hygromycin Untagged N/A
pReceiver-M68 CMV Mammalian Puromycin Untagged N/A
pReceiver-M29 CMV Mammalian Neomycin N-eGFP N/A
pReceiver-M03 CMV Mammalian Neomycin C-eGFP N/A
pReceiver-M98 CMV Mammalian Neomycin C-eGFP(monomeric) N/A
pReceiver-M15 CMV Mammalian Neomycin N-eYFP N/A
pReceiver-M16 CMV Mammalian Neomycin C-eYFP N/A
pReceiver-M32 CMV Mammalian Neomycin N-eCFP N/A
pReceiver-M33 CMV Mammalian Neomycin C-eCFP N/A
pReceiver-M04 CMV Mammalian Neomycin N-GST EK
pReceiver-M05 CMV Mammalian Neomycin N-Avi N/A
pReceiver-M48 CMV Mammalian Neomycin N-Avi+IRES-Biotin ligase N/A
pReceiver-M17 CMV Mammalian Neomycin C-Avi N/A
pReceiver-M06 CMV Mammalian Neomycin N-3xHA N/A
pReceiver-M07 CMV Mammalian Neomycin C-3xHA N/A
pReceiver-M08 CMV Mammalian Neomycin C-3xHA-His N/A
pReceiver-M43 CMV Mammalian Neomycin N-Myc N/A
pReceiver-M45 CMV Mammalian Neomycin C-3xHA+IRES-eGFP N/A
pReceiver-M09 CMV Mammalian Neomycin C-Myc N/A
pReceiver-M10 CMV Mammalian Neomycin C-Myc-His N/A
pReceiver-M72 CMV Mammalian Neomycin C-Myc+IRES2-eGFP N/A
pReceiver-M11 CMV Mammalian Neomycin N-Flag N/A
pReceiver-M12 CMV Mammalian Neomycin N-3XFlag N/A
pReceiver-M13 CMV Mammalian Neomycin C-Flag N/A
pReceiver-M46 CMV Mammalian Neomycin C-Flag+IRES-eGFP N/A
pReceiver-M14 CMV Mammalian Neomycin C-3XFlag N/A
pReceiver-M49 CMV Mammalian Neomycin N-HaloTag Tev protease
pReceiver-M50 CMV Mammalian Neomycin C-HaloTag Tev protease
pReceiver-M55 CMV Mammalian Neomycin N-mCherry N/A
pReceiver-M56 CMV Mammalian Neomycin C-mCherry N/A
pReceiver-M61 CMV Mammalian Neomycin IRES2-eGFP N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Lv105 CMV Lentiviral Puromycin Untagged N/A
pReceiver-Lv151 CMV Lentiviral Neomycin Untagged N/A
pReceiver-Lv152 CMV Lentiviral Hygromycin Untagged N/A
pReceiver-Lv153 CMV Lentiviral N/A Untagged N/A
pReceiver-Lv156 EF1a Lentiviral Puromycin Untagged N/A
pReceiver-Lv186 CMV Lentiviral N/A C-3xHA N/A
pReceiver-Lv157 CMV Lentiviral Neomycin C-3xHA N/A
pReceiver-Lv120 CMV Lentiviral Puromycin C-3xHA N/A
pReceiver-Lv108 CMV Lentiviral Puromycin N-Avi N/A
pReceiver-Lv124 CMV Lentiviral Puromycin C-Avi N/A
pReceiver-Lv242 CMV Lentiviral Puromycin C-Avi-FLAG N/A
pReceiver-Lv154 CMV Lentiviral Neomycin C-eCFP N/A
pReceiver-Lv127 CMV Lentiviral Puromycin C-eCFP N/A
pReceiver-Lv109 CMV Lentiviral Puromycin N-eCFP N/A
pReceiver-Lv103 CMV Lentiviral Puromycin N-eGFP N/A
pReceiver-Lv122 CMV Lentiviral Puromycin C-eGFP N/A
pReceiver-Lv182 CMV Lentiviral Neomycin N-eGFP N/A
pReceiver-Lv183 CMV Lentiviral Neomycin C-eGFP N/A
pReceiver-Lv189 CMV Lentiviral N/A N-eGFP N/A
pReceiver-Lv123 CMV Lentiviral Puromycin C-eYFP N/A
pReceiver-Lv104 CMV Lentiviral Puromycin N-eYFP N/A
pReceiver-Lv187 CMV Lentiviral N/A C-FLAG N/A
pReceiver-Lv101 CMV Lentiviral Neomycin N-Flag N/A
pReceiver-Lv121 CMV Lentiviral Puromycin C-FLAG N/A
pReceiver-Lv102 CMV Lentiviral Puromycin N-Flag N/A
pReceiver-Lv158 CMV Lentiviral Neomycin C-FLAG N/A
pReceiver-Lv203 CMV Lentiviral Puromycin C-Flag-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv216 CMV Lentiviral Puromycin C-Flag-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv129 CMV Lentiviral Puromycin C-Halotag Tev
pReceiver-Lv110 CMV Lentiviral Puromycin N-Halotag Tev
pReceiver-Lv211 CMV Lentiviral Hygromycin C-Halotag-IRES-hygromycin N/A
pReceiver-Lv128 CMV Lentiviral Puromycin C-His N/A
pReceiver-Lv215 CMV Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv205 CMV Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv220 CMV Lentiviral Hygromycin IRES2-Hygromycin N/A
pReceiver-Lv214 CMV Lentiviral N/A IRES2-mCherry N/A
pReceiver-Lv213 CMV Lentiviral Puromycin IRES2-mCherry-IRES-puromycin N/A
pReceiver-Lv111 CMV Lentiviral Puromycin N-mCherry N/A
pReceiver-Lv130 CMV Lentiviral Puromycin C-mCherry N/A
pReceiver-Lv155 CMV Lentiviral Neomycin C-mCherry N/A
pReceiver-Lv107 CMV Lentiviral Puromycin N-Myc N/A
pReceiver-Lv125 CMV Lentiviral Puromycin C-Myc N/A
pReceiver-Lv126 CMV Lentiviral Puromycin C-Myc-His N/A
pReceiver-Lv202 CMV Lentiviral Puromycin C-Myc-SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv204 CMV Lentiviral Puromycin C-Myc-SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv207 CMV Lentiviral Hygromycin SV40-eGFP-IRES-hygromycin N/A
pReceiver-Lv201 CMV Lentiviral Puromycin SV40-eGFP-IRES-puromycin N/A
pReceiver-Lv217 CMV Lentiviral Puromycin SV40-hLUC-IRES-puromycin N/A
pReceiver-Lv206 CMV Lentiviral Puromycin SV40-mCherry-IRES-puromycin N/A
pReceiver-Lv165 EF1a Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv166 EF1a Lentiviral N/A IRES2-mcherry N/A
pReceiver-Lv224 EF1a Lentiviral Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv225 EF1a Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pReceiver-Lv228 PGK Lentiviral N/A IRES2-eGFP N/A
pReceiver-Lv229 PGK Lentiviral N/A IRES2-mcherry N/A
pReceiver-Lv230 PGK Lentiviral Puromycin IRES2-mcherry-IRES-puromycin N/A
pReceiver-Lv231 PGK Lentiviral Puromycin IRES2-eGFP-IRES-puromycin N/A
pLX304 CMV Lentiviral (ORFeome) Blasticidin C-V5 N/A
EZShuttle™ Gateway® PLUS (1 vector) 
Vector Promoter Host Cell Selection Marker Tag Protease Site
pShuttle™ - Gateway PLUS shuttle N/A N/A
pShuttle ™ - Gateway PLUS shuttle
(no stop codon)
N/A N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-B01 T7 Bacterial N/A N-His N/A
pReceiver-B31 T7 Bacterial N/A C-His N/A
pReceiver-B02 T7 Bacterial N/A Untagged N/A
pReceiver-B03 T7 Bacterial N/A N-GST Tev
pReceiver-B04 T7 Bacterial N/A N-GST EK
pReceiver-B05 Tac Bacterial N/A N-GST Tev
pReceiver-B06 Tac Bacterial N/A N-GST EK
pReceiver-B07 Tac Bacterial N/A N-MBP Tev
pReceiver-B08 Tac Bacterial N/A N-MBP EK
pReceiver-B09 T7 Bacterial N/A N-Avi N/A
pReceiver-B10 Tac Bacterial N/A N-Flag N/A
pReceiver-B11 Tac Bacterial N/A N-His N/A
pReceiver-B12 Tac Bacterial N/A HisSUMO Sumo protease
pReceiver-B13 T7 Bacterial N/A HisSUMO SUMO Protease
pReceiver-B80 T7 Bacterial N/A N-Halo Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-Y01 GAL1 Yeast N/A C-His N/A
pReceiver-YAD pADH Yeast N/A GAL4AD N/A
pReceiver-YBD pADH Yeast N/A GAL4DB N/A
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-I01 AcMNPV polyhedrin Insect cell N/A N-His Tev
Vector Promoter Host Cell Selection Marker Tag Protease Site
pReceiver-WG02 T7 cell free N/A N-His Factor Xa
pReceiver-WG03 T7 cell free N/A N-HisSUMO CoolCutter(TM)
pReceiver-WG04 T7 cell free N/A N-AviSUMO CoolCutter(TM)
pReceiver-WG05 T7 cell free N/A N-HisAviSUMO CoolCutter(TM)
pReceiver-WG09 T7 cell free N/A HisGST TEV
pReceiver-WG16 T7 cell free N/A N/A N/A
pReceiver-WG31 T7 cell free N/A N-HisSUMOAvi CoolCutter(TM)
pReceiver-WG33 T7 cell free N/A N-TrxHisSUMO CoolCutter(TM)
 

 

 

Genome-wide human and mouse expression-ready ORF cDNA clones with N-terminal, C-terminal fusion tags or un-tagged.

 

Largest number of tags to choose from

Fluorescent tags eGFP, eYFP, eCFP, mCherry
Multifunctional tags HaloTag®, AviTag™
Solubility and purification tags His, SUMO, GST, MBP, Flag, 3xFlag
Antibody Immunoprecipitation tags HA, cMyc

Anti-tag antibodies available for easy detection

Popular tags and technologies

 
  • Lentiviral technology — Enables high efficiency gene delivery to virtually all cell types and whole model organisms resulting in high expression levels of delivered genes.
  • Internal ribosome entry site (IRES) technology — Allows coordinated and efficient co-expression of two genes with the same promoter in a single vector. When reporter and target genes are simultaneously expressed, biological activities of the target proteins are minimally affected.
  • HaloTag® technology — This novel multi-functional protein tag binds covalently and specifically to a variety of synthetic ligands, enabling tagged proteins to be labeled with fluorophores for in vitro or in vivo imaging or with affinity agents for purification.
  • AviTag™ technology — Based on site-specific in vitro or in vivo biotinylation of a short tag sequence enabling specific or reversible binding of avidin or streptavidin to biotin for immobilization, purification and visualization of the tagged target proteins.
  • Cell-free technology — Enables high success rate of target protein expression and natural protein folding with high percentage of active proteins. Proteins that are potentially toxic to cells can also be produced using the cell free system.

 

 

Custom Services

ORF mutant clones

Special promotion – Receive a free wild type ORF clone when you purchase a mutant ORF clone**.

Send your gene information and service requirement to inquiry@genecopoeia.com to request a quote.

 

ORF cDNA lentivirus 

  • High titer (guaranteed titer > 10^8 TU/ml)
  • Fast turnaround (2-4 weeks)
  • No additional cloning fees

 

Custom cDNA clone services Request a quote

  • Fast turnaround custom gene synthesis services
  • Competitive pricing

 

** Mutant ORF clone service is limited to site mutations, insertions and deletion services. Promotion expires December 31st, 2017. 

Clone Sets

ORF clone sets

Besides individual clones, GeneCopoeia also offers pre-made or custom-selected ORF cDNA clone sets at attractive prices. Uniformity and high-quality clone sets make them ideal for large-scale high content and high-throughput screening studies in functional genomics, proteomics and systems biology.

To learn more about our ORF cDNA clone sets, please contact us or call 866-360-9531.

 

Vector choices

Vector Vector Type Promoter Selection Marker Tag
pReceiver-M02 Mammalian expression CMV Neomycin N/A
pReceiver-M13 Mammalian expression CMV Neomycin C-Flag
pReceiver-M98 Mammalian expression CMV Neomycin C-eGFP (monomeric)
pReceiver-Lv105 Mammalian expression (lentiviral) CMV Neomycin N/A
pReceiver-Lv242 Mammalian expression (lentiviral) CMV Puromycin C-Avi-Flag
pDONR™ Vector Gateway shuttle N/A N/A N/A

Check out more vector types of your choice if none of the above meets your needs.

 

Clone sets by gene family in the vector of your choice

Please contact us at 1-866-360-9531 or inquiry@genecopoeia.com if you have any questions.

Vector:

More vector types are available

 

Cat. No. Species Set type Gene Clone Vector Vector type Price
OC-HCD-M02 Human CDs 341 430 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HCC-M02 Human cytokines and cytokine receptors 347 420 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HDG-M02 Human Drug target genes 5022 6144 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HGR-M02 Human GPCRs 526 583 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHI-M02 Human Histones 53 54 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHM-M02 Human Histone modification enzymes 65 87 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HII-M02 Human Innate immune proteins 565 702 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HIC-M02 Human Ion channels 297 366 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HKI-M02 Human Kinases 707 940 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HMP-M02 Human Membrane bound proteins 3567 4349 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HNH-M02 Human Nuclear hormone receptors 56 82 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPH-M02 Human Phosphatases 213 261 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPR-M02 Human Protease 404 488 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPI-M02 Human Protease inhibitors 111 134 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HSP-M02 Human Secretory proteins 1497 1788 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
OC-HTF-M02 Human Transcription factors 1309 1544 pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
Custom order Various Custom-selected Various Various pReceiver-M02
Non-viral
Expression-ready
Non-viral
Inquire
Cat. No. Species Set type Gene Clone Vector Vector type Price
OC-HCD-M13 Human CDs 349 430 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HCC-M13 Human cytokines and cytokine receptors 380 441 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HDG-M13 Human Drug target genes 5079 6035 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HGR-M13 Human GPCRs 515 554 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHI-M13 Human Histones 70 71 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHM-M13 Human Histone modification enzymes 67 101 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HII-M13 Human Innate immune proteins 67 101 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HIC-M13 Human Ion channels 290 332 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HKI-M13 Human Kinases 704 888 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HMP-M13 Human Membrane bound proteins 3389 3397 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HNH-M13 Human Nuclear hormone receptors 55 80 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPH-M13 Human Phosphatases 212 261 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPR-M13 Human Protease 429 498 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPR-M13 Human Protease inhibitors 115 133 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HSP-M13 Human Secretory proteins 1486 1736 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
OC-HTF-M13 Human Transcription factors 1454 1742 pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
Custom order Various Custom-selected Various Various pReceiver-M13
Non-viral
Expression-ready
Non-viral
Inquire
Cat. No. Species Set type Gene Clone Vector Vector type Price
OC-HCD-M98 Human CDs 345 425 pReceiver-M98 
Non-viral
Expression-ready
Non-viral
Inquire
OC-HCC-M98 Human cytokines and cytokine receptors 379 439 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HDG-M98 Human Drug target genes 5040 5979 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HGR-M98 Human GPCRs 513 550 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHI-M98 Human Histones 70 71 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HHM-M98 Human Histone modification enzymes 65 98 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HII-M98 Human Innate immune proteins 561 678 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HIC-M98 Human Ion channels 284 327 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HKI-M98 Human Kinases 696 869 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HMP-M98 Human Membrane bound proteins 3368 3969 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HNH-M98 Human Nuclear hormone receptors 54 78 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPH-M98 Human Phosphatases 210 260 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPR-M98 Human Protease 424 493 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HPI-M98 Human Protease inhibitors 113 129 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HSP-M98 Human Secretory proteins 1492 1737 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HSE-M98 Human Selenoprotein 33 38 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
OC-HTF-M98 Human Transcription factors 1455 1734 pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
Custom order Various Custom-selected Various Various pReceiver-M98
Non-viral
Expression-ready
Non-viral
Inquire
Cat. No. Species Set type Gene Clone Vector Vector type Price
OC-HCD-Lv105 Human CDs 325 406 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HCC-Lv105 Human cytokines and cytokine receptors 335 406 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HDG-Lv105 Human Drug target genes 4575 5556 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HGR-Lv105 Human GPCRs 352 391 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HHI-Lv105 Human Histones 51 52 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HHM-Lv105 Human Histone modification enzymes 67 91 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HII-Lv105 Human Innate immune proteins 545 674 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HIC-Lv105 Human Ion channels 236 283 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HKI-Lv105 Human Kinases 675 884 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HMP-Lv105 Human Membrane bound proteins 3154 3769 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HNH-Lv105 Human Nuclear hormone receptors 53 79 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HPH-Lv105 Human Phosphatases 202 247 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HPR-Lv105 Human Protease 379 447 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HPI-Lv105 Human Protease inhibitors 107 130 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HSP-Lv105 Human Secretory proteins 1441 1699 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
OC-HTF-Lv105 Human Transcription factors 1279 1522 pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
Custom order Various Custom-selected Various Various pReceiver-Lv105
Lentiviral
Expression-ready
Lentiviral
Inquire
Cat. No. Species Set type Gene Clone Vector Vector type Price
OC-HCD-GC Human CDs 323 422 pDONR™ Vector Gateway shuttle Inquire
OC-HCC-GC Human cytokines and cytokine receptors 346 423 pDONR™ Vector Gateway shuttle Inquire
OC-HDG-GC Human Drug target genes 4751 5836 pDONR™ Vector Gateway shuttle Inquire
OC-HGR-GC Human GPCRs 381 429 pDONR™ Vector Gateway shuttle Inquire
OC-HHI-GC Human Histones 37 37 pDONR™ Vector Gateway shuttle Inquire
OC-HHM-GC Human Histone modification enzymes 79 118 pDONR™ Vector Gateway shuttle Inquire
OC-HIC-GC Human Ion channels 257 312 pDONR™ Vector Gateway shuttle Inquire
OC-HII-GC Human Innate immune proteins 588 779 pDONR™ Vector Gateway shuttle Inquire
OC-HKI-GC Human Kinases 681 904 pDONR™ Vector Gateway shuttle Inquire
OC-HMP-GC Human Membrane bound proteins 3112 3576 pDONR™ Vector Gateway shuttle Inquire
OC-HNH-GC Human Nuclear hormone receptors 58 97 pDONR™ Vector Gateway shuttle Inquire
OC-HPH-GC Human Phosphatases 201 244 pDONR™ Vector Gateway shuttle Inquire
OC-HPI-GC Human Proteases inhibitor 111 137 pDONR™ Vector Gateway shuttle Inquire
OC-HPR-GC Human Protease 506 594 pDONR™ Vector Gateway shuttle Inquire
OC-HSP-GC Human Secretory proteins 1315 1472 pDONR™ Vector Gateway shuttle Inquire
OC-HTF-GC Human Transcription factors 1334 1695 pDONR™ Vector Gateway shuttle Inquire
Custom order Various Custom-selected Various Various pDONR™ Vector Gateway shuttle Inquire

 

Questions? Please submit your inquiry here or email us at inquiry@genecopoeia.com.

 

 

Related Clones

Related ORF Clones

EZShuttle™ Gateway® PLUS ORF cDNA shuttle clones – These are fully sequence-verified Gateway® ORF entry clones. These clones are compatible with both recombination and traditional cloning using multiple cloning sites (MCS).

ORFeome Collaboration cDNA clones – These are third-party constructed and sequence-verified Gateway® ORF entry clones enabling transfer into Gateway® compatible destination vectors.

Complete ORF cDNA clone collection and selection guide – Include comparison of ORF expression-ready clones, ORF Gateway® PLUS shuttle clones, ORFeome collaboration Gateway® entry clones and full-length cDNA clones. 

Control clones – Include positive and negative control clones that can be purchased together with or separately from your clone of interest. 

FAQs

Frequently Asked Questions: ORF clones

Answer: All you need to do is go to the ORF clones search page, search for your gene, and then choose the appropriate clones that will work for your system. If you have any custom requirements, then you will need to contact us and, after determining what you need, we will send you a custom quote.
Answer: We provide most ORF clones as 10 µg of purified plasmid. For our NextDay™ clones, you can choose between bacterial stock or transfection-ready DNA (10 µg or 50 µg). You can also request alternative delivery formats by contacting us at support@genecopoeia.com.
Answer: A NextDay™ ORF clone is a pre-made ORF clone. It is available at a competitive and ships next day if the order is placed by noon Eastern Time.
Answer: The turnaround time for ORF clones varies greatly, depending on many factors. If you order a NextDay™ clone in bacterial stock delivery time, shipping occurs the next day if the order is placed by noon Eastern Time. Most other clones have a turnaround time of about 2-3 weeks. If we need to make any customizations, or if we encounter any unforseen technical difficulties, the turnaround time could be longer.
Answer: When you order an OmicsLink™ Expression Ready ORF clone in one of our more than 150 vector types, you can order the same ORF cloned into a different vector type at a reduced price.
Answer: Once the clone is constructed, GeneCopoeia follows a rigorous quality control process to ensure the correct clone is delivered to you. We first amplify the sequences by PCR and validate the size. Next, all ORFs are fully sequenced. Restriction enzyme digestion is used to check the integrity of the entire plasmid.
Answer: Yes. pShuttle™ cloning vectors are available for DIY cloning using the EZShuttle™ BP Recombination Cloning System. (http://www.genecopoeia.com/category/product/cloning_products/).
Answer: A full-length ORF (Open Reading Frame) clone is a plasmid that contains a protein coding DNA insert that encodes a full-length protein. The DNA insert contains only the protein coding sequence (from start codon to stop codon) of a full-length gene (or cDNA) without 5' and 3' end untranslated regions (UTRs), or introns.
Answer: Due to variations between naturally-occurring protein-coding sequences and the NCBI reference sequence, GeneCopoeia does not guarantee a 100% match of OmicsLinkTM ORF clone sequences with NCBI RefSeq. GeneCopoeia guarantees that our OmicsLinkTM ORF Expression clone sequences are free of artificial frame-shifting variations, including deletionss and additionss of bases, as well as translation termination mutations (non-stop codon to stop codon) resulting from point mutation. This policy does not cover point variations due to possible naturally occurring polymorphisms and/or mutations that may be introduced by PCR in very rare cases. If you want a specific version of a gene from different cDNA libraries, GeneCopoeia will make its best effort to obtain that particular version but without guarantee of success.
Answer: The expression system should be specified first. We recommend the lentiviral system if the mammalian target cell is hard to transfect. Next, tags should be chosen. GeneCopoeia provides fluorescent tags, solubility and purification tags, antibody immunoprecipitation tags and multifunctional tags.
Answer: Tags should be placed far from the active center and functional domains of the target protein. If the target protein has an N-terminal signal peptide, then the tag must be placed at the C terminus.
Answer: EZShuttle™ Gateway® PLUS ORF clones cannot express proteins in GeneCopoeia’s pShuttle™ vector. However protein-coding ORFs in these clones can be transferred into any compatible GeneCopoeia pEZ™ expression cloning or Gateway® destination expression vector simply and rapidly. These new vectors can then be used to express the desired protein in the targeted cell type.
Answer: This information can be found in the clone datasheet you received.
Answer: GeneCopoeia provides a suite of pEZ™ expression cloning vectors (similar to pDEST destination vectors  from ThermoFisher Scientific) that can produce various forms of proteins (such as native, N-terminal fusion or C-terminal fusion) in different host cell systems including bacterial, yeast, insect and mammalian cell systems. EZShuttle™ Gateway® PLUS ORF clones can also be used to transfer ORFs to ThermoFisher Scientific's pDEST destination vectors.
 
Answer: Currently, we offer pre-made human, mouse, and zebrafish ORF clones. We are in the process of producing full length ORF clones from other model animals such as rat. These full-length ORF clones will be released to market in the near future. If you are interested in a full length ORF of any genes of interest from other model organisms, please send us the information of these genes, such as GenBank Accession or Nucleotide sequences. They will be charged with the same price as our other catalog ORF clones. We also offer custom cloning services for cloning genes from other model organisms into any vector of your choice.
Answer: A correct DNA sequence with a promoter does not guarantee you will get protein expressed. Many factors contribute to the success or failure of protein expression: 1) Did you use the right type of expression vector for your expression host system? For instance, our pReceiver-M01 vector is for mammalian cell expression and pReceiver-B01 is for bacterial expression. 2) If the protein is a membrane protein, does it need target sequence for proper localization? 3) Will the over-expressed protein be toxic to the host? 4) Will the protein be folded into its correct three-dimensional structure for proper function and/or immunological assays? 5) Have you checked the sequencing to see if you get an alternative splicing variant?
Answer: All expression clones in pReceiever-B01 and pReceiver-WG02/WG03/WG04/WG05/WG09/WG16 have been tested for expression in E. coli or the cell free protein production system made by Roche. Other expression clones with different features and for different host cell systems have not been tested for protein expression.
Answer: We will make the best effort to clone the original splicing form and provide you with the replacement clone. However, we cannot guarantee that we will get the clone of a specific splicing variant.
 
Answer: Even though we cannot provide replacement clones for this type of case, we do offer mutagenesis services to change any nucleotide composition as requested for a nominal fee.
 
Answer: We guarantee that there are no frame-shifting changes and no premature stop codons in our full length ORF inserts compared with reference sequences. We will replace defective clones or refund the charges if that occurs.
Answer: The differences can originate from two potential sources or a combination of the two: 1) Naturally occurring events including polymorphisms, alternative splicing, inconsistencies in multiple copies/versions of the same genes resulting from annotation updates, sequencing errors and/or ambiguities; 2) Artificially introduced changes caused by PCR cloning. Very often, potential users of our clones compare our sequencing data with the sequence of only one reference transcript, and attribute any discrepancies to PCR introduced mutations. Through analysis of multiple clones of close to 20,000 human genes, we found that the vast majority of discrepancies can be clearly attributed to category 1), when comparing our sequence data with all transcripts and sequences in the corresponding gene locus including genomic sequence, ESTs, other cDNA/mRNA transcripts. Typically, we found that there are fewer discrepancies between the sequences of our ORF clones and the corresponding genomic sequence than the sequences of public domain gene transcripts and the corresponding genomic sequences. We certainly cannot entirely eliminate the possibility of mutations introduced by our PCR cloning process, but we believe they are rare occurrences. We offer services at a small fee for changing base nucleotide compositions.
 
Answer: We use PCR together with several patented technologies to maximize the fidelity of PCR to generate full length ORFs (more information can be found in the ORF White Paper). We used more than 70 cDNA libraries of human or mice tissues as templates to generate full length ORFs.
Answer: For EZShuttle™ Gateway® PLUS ORF clones, the forward sequencing primer (from upstream of the 5' end of the ORF) is: 5'-CCCAGTCACGACGTTGTAAAACG, and the reverse sequencing primer (from downstream of the 3' end of the ORF) is: 5'-ATGGTCATAGCTGTTTCCTG. For  OmicsLink™ clones, sequencing primers are indicated in the datasheets for each individual clone. To download the datasheet, please visit the Technical Resources section on our website. Please note that you will need the following information in order to download your data sheets: Login information for your account on our website, the catalog number(s) of your clone(s), and your sales order number (SO#). If you are unable to download the datasheets from our website, please contact Technical support.
Answer: Each full length ORF insert is fully sequenced. Before the delivery of each clone, we re-check the clone by PCR with gene-specific primers to ensure that the correct clone is shipped.
Answer: Yes, we will clone the gene for you. It is our mission and continuing effort to clone all full-length ORFs of human genes. It is likely that the gene that you are interested in is already in our production pipeline or in the queue for future cloning. If you provide us with the GenBank accession number, gene name, nucleotide or amino acid sequences, we will place the gene in the list of priority genes to be cloned. Usually, it takes two weeks to complete the cloning, although we cannot guarantee that we will get the gene. You will be notified as soon as the clone becomes available. There will be no additional charge.
 
Answer: There are usually multiple gene transcripts in a gene locus, resulting from alternative splicing, updated information about ORFs, polymorphisms, or redundancies from duplication of cloning efforts from different laboratory groups. During the process of selecting full-length genes for ORF cloning, we try to choose the most representative transcript from each gene locus, which may differ slightly or significantly from other transcripts in the same gene locus. This approach provides the broadest coverage of all gene loci. In many instances, we do obtain multiple variants or versions of full length ORFs from our cloning production pipeline. Customers are encouraged to send inquires to inquiry@genecopoeia.com if he/she is interested in obtaining a particular variant/version of a gene/ORF.In some cases, discrepancies could originate from changed annotations of ORF start and/or stop locations as the result of newly available sequence information and/or experimental evidence. We regularly check for information updates on ORF annotations in public databases such as NCBI and EMBL. The new ORF annotations will then be used in our recloning efforts to keep our ORF clones updated.
 
Answer: No, the sequence on the website is not the actual sequence but the reference sequence from NCBI. The actual sequence of the clone may differ due to genetic variation and alternative splicing. The actual sequence for any of the ORF cDNA clones can be requested at any time which can be sent by e-mail. However, please note that the actual sequence for clones in pre-production or clones offered via Custom Gene Synthesis may not yet be available.
 

Answer: The sequences are different depending on which full length ORF clone for both EZShuttle™ Gateway® PLUS ORF clones and OmicsLink™ ORF Expression Clones you purchased. First, check the datasheet that is shipped with the clone and use the figure to identify the sequence.

Fig1. Example: Multiple cloning site of Catalog No. GC-B0128

Answer: OmicsLink™ vector backbones may contain one to two selection markers, one for bacterial selection and one for mammalian stable selection. There are various options for the selections. Please check the selection options carefully when you order OmicsLink™ Expression Clones.(http://www.genecopoeia.com/product/orf-clone/).
Answer: KAN.
Answer: GeneCopoeia's OmicsLink™ Expression Clones are generated by our patented RecJoin™ cloning technology. By using this technology to construct a 5' tag-ORF fusion expression clone, there is no attB site between the 5' tag and the start codon ATG of the full-length ORF. The extra 8 amino acids encoded by the attB site in ThermoFisher Scientific's pDEST expression clones will not be present in the proteins produced by using OmicsLink™ Expression clones. The same principle applies to 3' fusion protein production.
 
Answer: There are fourteen amino acids between the fusion peptide (or N-terminal tag) and the ORF encoded protein. These fourteen amino acids are, from N-terminus to C-terminus, Thr-Ser-Leu-Tyr-Lys-Lys-Ala-Gly-Leu-Gly- Gly-Val-Arg-Thr. Eight amino acids (Thr-Ser-Leu-Tyr-Lys-Lys-Ala-Gly) are encoded by the attB recombination site and six (Leu-Gly-Gly-Val-Arg-Thr) are encoded by the sequences between attB and the ATG start codon.
 
Answer: Each EZShuttle™ Gateway® OLUS ORF Clone has two multiple cloning sites (MCS) flanking the ORF. By choosing suitable restriction enzymes, you can cut out the entire ORF from EZShuttle™ Gateway® OLUS ORF Clones and insert the ORF into your vectors by classical sub-cloning methods.
Answer: You can make a point mutation to remove the stop codon. There are more than 35,000 full length ORFs that have been constructed with a tag at their 3' ends, such as eGFP and HA. There are more than 10 types from which you can choose (http://www.genecopoeia.com/tech/omicslink/). You can ask us to remove the stop codon and clone it into the vector(s) of your interest for a small fee.
 
Answer: Yes, and there are two choices. First, you can use the OmicsLink™ Expression Clones that we have developed. We have already constructed ORF clones in expression vectors with N-terminal tags or C-terminal tags, such as His tag and eGFP. Tthere are more than 10 types of tags to meet your needs. Please check the OmicsLink™ product information page (http://www.genecopoeia.com/tech/omicslink/). Second, you may also use GeneCopoeia's pEZ™ or ThermoFisher Scientific's Gateway® expression vectors for expressing N-terminal or C-terminal fusion proteins. Because our EZShuttle™ Gateway® OLUS ORF Clones contain stop codons, you will need to remove the stop codons before transferring ORF inserts into GeneCopoeia's pEZ™ or ThermoFisher Scientific's pDEST expression vectors to produce C-terminal tagged fusion proteins.
 
Answer: The selection procedure is very stringent. It includes extraction, comparison and validation of gene sequences and their annotation information from multiple public and private sources. Clustering and manual curation are applied to reduce redundancy and eliminate erroneous genes.
Answer: GeneCopoeia provides a suite of pEZ™ expression cloning vectors (similar to pDEST destination vectors  from ThermoFisher Scientific) that can produce various forms of proteins (such as native, N-terminal fusion or C-terminal fusion) in different host cell systems including bacterial, yeast, insect and mammalian cell systems.
 
Answer: The vector for EZShuttle™ Gateway® OLUS ORF Clones manufactured by GeneCopoeia harbors full length ORFs flanked by attL1 and attL2 recombination sites that pair with the Gateway® pDEST expression vectors containing attR1 and attR2 recombination sites. When GeneCopoeia's EZShuttle™ Gateway® PLUS ORF Clones are mixed with Gateway® compatible pDEST expression vector(s), with optimal concentrations of enzymes and conditions, the ORFs in the EZShuttle™ Gateway® PLUS ORF Clones™ can be transferred into Gateway® pDEST expression vector(s). Because of the unique feature of recombination sites in the vectors, the transferred ORFs will be under the control of the promoter(s) in the destination expression vector(s).
 
Answer: The full-length ORF clones contain only the protein coding sequences, while other full-length whole transcript cDNA clones contain non-coding sequences, such as 5' and/or 3' UTRs, which have been known to have possible negative impacts on the protein translation process. Please visit this page for further details on the comparison of these two types of clones: (http://www.genecopoeia.com/tech/application/).
Answer: We recommend that you refer to the product by its catalog number (eg: EX-V0379-M29) and refer to us as GeneCopoeia, Inc. (Rockville,MD).

*30% off ORF clone promotion is valid for selected ORF clones only. Offer is not valid for gene synthesis services, ORFeome clones, and custom services. Not valid for previous purchases. Offer expires October 31, 2017. Next day shipping is valid for Nextday-ORF clones only. Valid in US and Canada only.