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Nucleic Acid Quantitation

Introduction

GeneCopoeia iQuant™ DNA or RNA Quantitation Kits allow precise quantitation of DNA or RNA samples across a wide range of concentrations. Our new iQuant™ NGS dsDNA assay kits are ideal for quantitating DNA samples in Next Gen Sequencing applications. Unlike absorbance-based nucleic acid quantitation, fluorescence-based DNA or RNA quantitation is highly sensitive and selective for DNA or RNA, and provides  more accurate DNA or RNA concentration in the presence of common contaminants, including free nucleotides, protein, detergents and salts.

Features

  • Higher sensitivity: 100 times more sensitive than NanoDrop;
  • Broad dynamic range: over four orders of magnitude;
  • More accuracy: unaffected by the presence of proteins, free nucleotides;
  • Easy to use: mix-and-read format;
  • Instrument compatibility: assay can be performed using a fluorescence plate reader, or a fluorometer such as Qubit® or Quantus™.

DNA quantitation of purified DNA samples is a standard nucleic acid research workflow, providing confidence for downstream applications such as Next Gen Sequence, PCR, Cloning, and DNA transfections (Figure 1).


Figure 1. Nucleic acid research workflow

GeneCopoeia' iQuant™ NGS dsDNA assay kits provide a very simple workflow (Figure 2).


Figure 2. iQuant™ NGS dsDNA assay workflow

In addition, GeneCopoeia' iQuant™ DNA Quantitation Kits provide higher sensitivity than absorbance-based quantitation methods, such as NanoDrop (Figure 3).

                            
Figure 3. Improved sensitivity with iQuant™ DNA Quantitation Kits

Further, iQuant™ DNA Quantitation Kits exhibit excellent selectivity for DNA, even in the presence of an equal mass of RNA (Figure 4).


Figure 4. Selectivity for dsDNA using iQuant™ HS dsDNA Quantitation Kit in the presence of an equal mass of RNA.

iQuant™ DNA Quantitation Kits show superior accuracy for detecting dsDNA compared with NanoDrop, especially at relatively low DNA concentrations (Figure 5).


Figure 5. Comparison of accuracy of DNA quantitation between the iQuant™ HS dsDNA Quantitation Kit and NanoDrop.

The iQuant™ DNA kits for DNA quantitation are simple to use. Just mix your DNA sample with iQuant™ dye solution, and perform the assays in either a multi-well plate reader, or in single tubes (Figure 6). The iQuant™ kits are compatible with the Qubit® fluorometer from Life Technologies and the Quantus™ fluorometer from Promega.


Figure 6. Assay workflow for iQuant™ DNA Quantitation Kits

To order

iQuant™ Nucleic Acid Quantitation Selection Guide

  Target Initial Sample Concentration Application
iQuant™ HS dsDNA Quantitation Kit dsDNA 4 pg/µL – 100 ng/µL Useful for quantitation of PCR products, viral DNA, and samples for subcloning and Next Gen Sequencing
iQuant™ NGS-HS dsDNA Assay Kit dsDNA 4 pg/µL – 100 ng/µL Useful for quantitation of DNA samples for Next Gen Sequencing
iQuant™ BR dsDNA Quantitation Kit dsDNA 40 pg/µL – 1,000 ng/µL Useful for quantitation of genomic and miniprep DNA samples
iQuant™ NGS-BR dsDNA Assay Kit dsDNA 40 pg/µL – 1,000 ng/µL Useful for quantitation of DNA samples for Next Gen Sequencing
iQuant™ ssDNA Quantitation Kit ssDNA 20 pg/µL – 200 ng/µL Useful for quantitation of oligo, primers, denatured DNA, PCR products
iQuant™ HS RNA Quantitation Kit RNA 100 pg/µL – 100 ng/µL Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures
iQuant™ BR RNA Quantitation Kit RNA 400 pg/µL – 1,000 ng/µL Useful for quantitation of samples for microarray, RT-PCR, and Northern blot procedures
Buy Catalog # Product Name Sensitivity Unit Size List Price
N010 iQuant™ High Sensitivity dsDNA Reagent (200 X) 0.2-100 ng 1 mL $120
N011 iQuant™ High Sensitivity dsDNA Quantitation Kit 0.2-100 ng 1,000 assays $180
N012 iQuant™ Broad Range dsDNA Reagent (200 X) 2-1000 ng 1 mL $120
N013 iQuant™ Broad Range dsDNA Quantitation Kit 2-1000 ng 1,000 assays $180
N014 iQuant™ ssDNA Reagent (200 X) 1-200 ng 1 mL $120
N015 iQuant™ ssDNA Quantitation Kit 1-200 ng 1,000 assays $180
N016 iQuant™ High Sensitivity RNA Reagent (200 X) 5-100 ng 1 mL $120
N017 iQuant™ High Sensitivity RNA Quantitation Kit 5-100 ng 1,000 assays $180
N018 iQuant™ Broad Range RNA Reagent (200 X) 20-1000 ng 1 mL $120
N019 iQuant™ Broad Range RNA Quantitation Kit 20-1000 ng 1,000 assays $180

N020-1
N020-2
iQuant™ NGS-HS dsDNA Assay Kit 0.2-100 ng 250 assays
500 assays
$100
$160

N021-1
N021-2
iQuant™ NGS-BR dsDNA Assay Kit 2-1000 ng 250 assays
500 assays
$100
$160
N022 iQuant™ Assay Tubes   500 tubes $60