miExpress™ precursor microRNA expression clones

MicroRNAs (miRNAs) are small, non-coding single stranded RNA molecules found in eukaryotic organisms. They are highly conserved and usually 21-23 nucleotides in length. miRNAs are involved in almost all cellular functions and regulate gene expression by binding to the 3' untranslated regions (3' UTRs) of targeted mRNAs. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

GeneCopoeia offers miExpress precursor miRNA expression clones in lentiviral and non-viral vectors with eGFP reporter gene. Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.

Lentivirus for all known microRNA precursors is available as custom service for only $995 in 2-4 weeks. Search for your lentivirus here.

Figure 1. Vector backbones of miExpress precursor microRNA clones

Precursor miRNA vector features

Vector Reporter gene Selection marker Type Advantage pEZX-MR01 eGFP Neomycin FIV FIV based for transduction into difficult to target cells pEZX-MR04 eGFP Puromycin Mammalian Mammalian vector for transient or stable transfection into commonly used cell types	Need microRNA lentivirus? GeneCopoeia offers microRNA lentivirus as a custom service. Only $995 and delivered within 2-4 weeks.

Advantages Full coverage All known human, mouse and rat miRNAs in the current miRBase covered. Flexible delivery systems Lentiviral vectors allow efficient transduction and stable integration into the host genome of non-dividing and difficult to transfect cells target cells Non-viral vectors allow either transient or stable transfection Optimized expression cassettes Expression cassettes have been fully sequenced and optimized for high expression of precursor miRNA and maturation of miRNA inside cells Selection markers and reporters An eGFP reporter is designed to monitor transfection or transduction efficiency Neomycin or Puromycin selection marker enables stable cell line selection

Figure 2: Role of precursor miRNA and miRNA 3' UTR target clones in miRNA regulation studies

Related microRNA products
miTarget™ microRNA 3' UTR target clones — Dual lucifearse reporter vector-based miArrest™ microRNA Inhibitors — Synthetic and Lentiviral and non-viral vector-based All-in-One™ microRNA primers and qRT-PCR detection kit — SYBR Green based detection kits and pre-validated primers MicroRNA lentivirus — Request a quote for microRNA lentivirus production service		Publications using GeneCopoeia microRNA solutions