Luc-Pair™ Duo-Luciferase HT Assay Kit

*50% Off Luc-Pair™ Luciferase Kits

For High-Throughput Applications

Introduction

GeneCopoeia's Luc-Pair™ Duo-Luciferase HT Assay Kit is a convenient system for high-throughput detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activities in 96- or 384-well plates. The Luc-Pair™ Duo-Luciferase HT Assay reagents can be added directly to cells in growth medium without washing or preconditioning, and are optimized for use with many different media containing 0–10% serum (e.g. DMEM, RPMI1640, etc.) Firefly luciferase luminescence is produced by one reagent, while a second reagent simultaneously quenches the Firefly luciferase and produces Renilla luciferase luminescence. 

The Luc-Pair™ Duo-Luciferase HT Assay Kit works great for many different eukaryotic cell types (e.g. mammalian, invertebrates, etc.)  and is compatible with GeneCopoeia, Promega and many other Fluc and Rluc vectors.   

 

Advantages

· Enhanced stability. 
· Convenient — Directly lyse cells in culture medium and measure luciferase activities simultaneously. 
· Versatility — Works well with many different eukaryotic (adherent or suspended) cells using micro-plate luminescence readers.
· Low background — Very limited background luminescence. No subtraction from readings is required.
· Simplicity — Renilla luciferase buffer contains the quenchers for Firefly luciferase activity (Figure 3). enabling a quick Glow and Stop-N-Glow two-step assays.
· Reproducibility — Reliable, linear results for a concentration range over several orders of magnitude. 

 

Figure 1.  Activities of Firefly and Renilla luciferase signals using the GeneCopoeia (GCI) Luc-Pair Duo-Luciferase HT Assay Kit. HEK 293 cells were transfected with Promega pGL4.13/pGL4.75 reporter vectors for 48 hours. FLuc and RLuc activities were measured as described in the procedure. Promega’s Dual-Glo luciferase assay kit was used (FLuc-Prmg and RLuc-Prmg) in comparison.  

 

Figure 2. Firefly and Renilla luciferase activity mesured in various media. HEK 293 cells were transfected with Promega pGL4.13/pGL4.75 reporter vectors for 48 hours. 5×104 transfected cells were suspended in DMEM without serum or the following types of media containing 10% serum:  DMEM, RPMI1640, EMEM, IMDM, McCoy’s 5A, F-12K, MEBM, ACL-4, L15, Cho-S-SFMII, NCTC109. Firefly and Renilla luciferase activities were measured as described in the procedure.

Figure 3. Firefly luciferase activity is quenched with the addition of Luc-HT Buffer II. HEK 293 cells were transfected with Promega pGL4.13/pGL4.75 reporter vectors for 48 hours. Firefly luciferase activity was measured as described in the procedure. Next, 1× Luc-HT Buffer II (without or with substrate) was added to the wells, followed by count reading in a luminometer. About 99.9% of firefly luciferase activity was quenched (middle column).

To Order

Luc-Pair™ Duo-Luciferase HT Assay Kit

Buy Catalog# Product Description Price
LF013 Luc-Pair™ Duo-Luciferase HT Assay Kit (10 ml) For high-Throughput detection of Firefly and Renilla luciferase activities. $156
LF014 Luc-Pair™ Duo-Luciferase HT Assay Kit (30 ml) $375
LF015 Luc-Pair™ Duo-Luciferase HT Assay Kit (100 ml) $1022

  User manual MSDS

 

Vector options (Discounted price – $199)

Buy Catalog# Product Description Promoter Reporter gene Tracking gene Selection marker Size Price
ZX001 pEZX-FR01 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector N/A* Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin 10µg $495
ZX002 pEZX-FR02 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector SV40 Firefly luciferase (hLuc) Renilla luciferase (Rluc) N/A* 10µg $495
ZX003 pEZX-FR03 Firefly luciferase (FLuc) and Renilla luciferase (Rluc) reporter cloning vector miniCMV Firefly luciferase (hLuc) Renilla luciferase (Rluc) Puromycin 10µg $495

 

 

*Discount offer is valid for 30ml and 100ml only. Not valid for previous purchases. Offer expires June 30th, 2017.