Introduction The Gateway® Cloning Technology is based on the site specific recombination reaction of phage lambda, an efficient biochemical process that conserves genetic information. It is a new universal system for cloning and subcloning DNA sequences, to facilitate the functional analysis of genes and protein expression. LR Recombinase Enzyme Mix contains Int (λ Integrase), IHF (E.coli Integration Host Factor) and Xis (Excisionase protein) that catalyze the recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone in vitro. LR Recombinase Enzyme Mix Kit is the enzyme and buffer in separate tubes.

Components Supplied •  LR Recombinase Enzyme Mix •   5X Reaction Buffer •   Positive Control (100ng/µl ) Figure 1  The LR reaction。attL and attR recombine to form a co-intergrate.