Lentifect™ Crude and Purified Lentiviral Particles — high titers and ready to transduce
Lentifect™ Crude and Purified Lentivirus
Lentiviral vectors provide potent vehicles for delivery of genes into a wide range of cell types including difficult-to-transfect cells. Lentiviral particles transduce cells and integrate into the host genome in dividing and post-mitotic cells resulting in long-term expression of the transgene both in vivo and in vitro. Producing, concentrating and titrating lentiviral particles, however, is time consuming and requires some experience to achieve high titers and consistent results. So, GeneCopoeia provides efficient scientific expertise for reliable production of viral particles.
Lentifect™ crude and purified lentivirus are available as both pre-made and custom-made. High titer lentivirus are produced from standardized protocols using high-purity plasmid DNA preparations and EndoFectin™ Lenti transfection and TiterBoost™ reagents. The high quality reagents and GeneCopoeia scientific expertise ensure production of the highest-titers possible of crude viral supernatant and purified particles.
Pre-made lentivirus
Pre-made crude and purified lentivirus are immediately available for the following categories including
Commonly used lentivirus — CRE recombinase, FLP recombinase, SV40 large T antigen, HIV tat
Negative controls
Pre-made lentivirus titer ranges from 10^7 copies/ ml for crude version to 10^9 copies/ ml for purified lentivirus. Lentifect lentivirus production process involves stringent quality control processes including sequence verification of the insert, qRT-PCR based titer estimation, etc. View transduction results of H1299 cells with Lentifect eGFP or mCherry lentivirus.
Special collection lentivirus
- Low price and fast delivery
Special collection of lentiviral particles include:
Genes:
Human ORF cDNAs in OmicsLink™ pReceiver-Lv105 lentiviral vector
miRNAs:
All known human, mouse and rat miRNAs in miExpress™ pEZX-MR03 lenviral vector
miRNA inhibitors:
All known human, mouse and rat miRNA inhibitors in miArrest™ pEZX-AM03 lentiviral vector
Cancer-related miRNAs and inhibitors:
Human cancer-related miRNAs and inhibitors in pEZX-MR03 and pEZX-AM03 lenviral vectors respectively
The delivery time is around 2-4 weeks. For lentiviral particles not covered in this collection, please check out our Custom-made and pre-made lentivirus.
Custom-made lentivirus
Custom lentivirus production services are offered for any construct including ORF cDNA, shRNA, miRNA precursors and miRNA inhibitors. A wide range of expression vectors with and without fusion tags are available for ORF cDNA, shRNA and miRNA. GeneCopoeia' s scientific expertise can deliver high titer lentivirus in either crude or purified form with titers ranging from 10^7 copies/ ml to 10^10 copies/ ml depending upon the request.
Delivery estimate typically ranges from two to few weeks depending upon the nature of the custom request. Lentivirus can be delivered in different sizes including 200 µl and 1 ml.
Ready-for-transduction, Lentifect lentivirus provide the following advantages:
A cost-effective choice for busy labs
A reliable lentiviral source for consistent quality from lot to lot
Efficient transduction of difficult-to-transfect cells or animal models
Transduction of H1299 cells with GeneCopoeia eGFP lentiviral particles
Panel A. Numbers correspond to the volume of crude eGFP particles used.
Panel B. Numbers correspond to the volume of purified eGFP particles used.
Figure 1. H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of eGFP either crude lentiviral particles, catalog number LP-EGFP-LV105 (Panel A) or purified particles, catalog number LPP-EGFP-LV105 (Panel B) in the presence of 5 µg/ml of polybrene. The expression of eGFP was checked with a fluorescence microscope 72 hours post-transduction.
Transduction of H1299 cells with GeneCopoeia mCherry lentiviral particles
Figure 2. H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of crude mCherry lentiviral particles, catalog number LP-MCHR-LV105 in the presence of 5 µg/ml of polybrene. The expression of mCherry was checked with a fluorescence microscope 72 hours post-transduction.
