Lentifect™ Crude and Purified Lentiviral Particles — high titers and ready to transduce
Lentiviral vectors provide potent vehicles for delivery of genes into a wide range of cell types including difficult-to-transfect cells. Lentiviral particles transduce cells and integrate into the host genome in dividing and post-mitotic cells resulting in long-term expression of the transgene both in vivo and in vitro.
Producing, concentrating and titrating lentiviral particles, however, is time consuming and requires some experience to achieve high titers and consistent results.
When resources are limited, or accelerating your research is a top priority, GeneCopoeia provides efficient scientific expertise for reliable production of viral particles. Several popular varieties are already in stock.
Transduction of H1299 cells with GeneCopoeia eGFP lentiviral particles
Panel A. Numbers correspond to the volume of crude eGFP particles used.
Panel B. Numbers correspond to the volume of purified eGFP particles used.
Figure 1. H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of eGFP either crude lentiviral particles, catalog number LP-EGFP-LV105 (Panel A) or purified particles, catalog number LPP-EGFP-LV105 (Panel B) in the presence of 5 µg/ml of polybrene. The expression of eGFP was checked with a fluorescence microscope 72d hours post-transduction.
GeneCopoeia produces crude and purified lentiviral particles for the following nucleic acids:
shRNA
miRNA
full-length ORF cDNA genes
iPS reprogramming genes
Fresh high-titer stocks ready to transduce
GeneCopoeia lentiviral particles are produced from standardized protocols using high-purity plasmid DNA preparations and EndoFectin™ Lenti transfection and TiterBoost™ reagents ensuring production of the highest-titers possible of crude viral supernatant and purified particles ever achieved. Ready-for-transduction, lentiviral preps from GeneCopoeia provide the following advantages:
A cost-effective choice for busy labs
A reliable lentiviral source for consistent quality from lot to lot
Efficient transduction of difficult-to-transfect cells or animal models
Ready-to-transduce lentiviral particles from GeneCopoeia provide an economical and time-saving solution for labs that wish to fast-track their research and/or lack the skill and materials required to produce lentiviral particles efficiently.
GeneCopoeia has several lentiviral particles ready-to-go. Custom orders take two to six weeks to fill.
Please inquire for custom-made particles and/or download general instructions
Lentifect™ Purified Lentiviral Particles(see table below for crude particles)
Transduction of H1299 cells with GeneCopoeia mCherry lentiviral particles
Figure 2. H1299 cells (ATCC) in 24-well plates were transduced with the indicated amounts of crude mCherry lentiviral particles, catalog number LP-MCHR-LV105 in the presence of 5 µg/ml of polybrene. The expression of eGFP was checked with a fluorescence microscope 72d hours post-transduction.
Lentifect™ Crude Lentiviral Particles (see table above for crude particles)
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