GeneCopoeia‘s GLuc-ON™ GR transcriptional response element (TRE) clone enables analysis of gene activation in response to glucocorticoid receptor activity. The glucocorticoid receptor is a transcription factor that is transported to the nucleus in response to steroid hormone binding, and is involved in many physiological processes, such as stress, lipid metabolism, and inflammation.
The GLuc-ON™ GR TRE clone contains tandem repeats of GR upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells. The GR response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ GR TRE clone is provided as transfection-ready DNA. Negative (non-activating), positive (constitutively-activted) and normalization (secreated alkaline phosphatase) control clones are also available for purchase.
Figure 1. Activation of the GLuc-ON™ GR TRE by dexamethasone (DEX). 293T cells were transfected with a negative control clone or the GLuc-ON™ GR TRE clone. Transfected cells were incubated for 24 hours, followed by 18 hours of treatment with DEX. A Gaussia Luciferase assay was performed, and transcriptional response activity values are expressed as luminescence fold activation.