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VividFISH Chromosome FISH Probes

Bright fluorescent probes for chromosome enumeration and controls for locus-specific detection.

Introduction

 

GeneCopoeia's VividFISH™ fluorescence in situ hybridization (FISH) chromosome probes are the ideal tools for chromosome detection. FISH chromosome probes are extremely valuable for many applications, including:

  • As control probes to accompany disease gene-specific (LSI) FISH probes.
  • As chromosome enumeration probes (CEP), to be used to determine chromosome copy number in diagnosis and prognosis of cancers and other diseases.
  • As chromosome enumeration probes for determining target chromosome copy number in CRISPR- and TALEN-mediated genome editing projects.

Advantages

  • Exceptionally bright fluorescent probes make chromosome detection easy and convenient.
  • Each probe available for fluorescence in multiple colors.
  • Great for use in chromosome copy number determination for genome editing applications.

 

Order VividFISH™ chromosome enumeration probes (CEP)

Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP001 FISH CEP probe for human chromosome 1-green Green 10 hybridizations $225
FP002 FISH CEP probe for human chromosome 1-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP006 FISH CEP probe for human chromosome 2-green Green 10 hybridizations $225
FP007 FISH CEP probe for human chromosome 2-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP011 FISH CEP probe for human chromosome 3-green Green 10 hybridizations $225
FP012 FISH CEP probe for human chromosome 3-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP016 FISH CEP probe for human chromosome 4-green Green 10 hybridizations $225
FP017 FISH CEP probe for human chromosome 4-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP021 FISH CEP probe for human chromosome 5-green Green 10 hybridizations $225
FP022 FISH CEP probe for human chromosome 5-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP025 FISH CEP probe for human chromosome 6-green Green 10 hybridizations $225
FP026 FISH CEP probe for human chromosome 6-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP031 FISH CEP probe for human chromosome 7-green Green 10 hybridizations $225
FP032 FISH CEP probe for human chromosome 7-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP036 FISH CEP probe for human chromosome 8-green Green 10 hybridizations $225
FP037 FISH CEP probe for human chromosome 8-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP041 FISH CEP probe for human chromosome 9-green Green 10 hybridizations $225
FP042 FISH CEP probe for human chromosome 9-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP046 FISH CEP probe for human chromosome 10-green Green 10 hybridizations $225
FP047 FISH CEP probe for human chromosome 10-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP051 FISH CEP probe for human chromosome 11-green Green 10 hybridizations $225
FP052 FISH CEP probe for human chromosome 11-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP056 FISH CEP probe for human chromosome 12-green Green 10 hybridizations $225
FP057 FISH CEP probe for human chromosome 12-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP061 FISH CEP probe for human chromosome 13-green Green 10 hybridizations $225
FP062 FISH CEP probe for human chromosome 13-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP066 FISH CEP probe for human chromosome 14-green Green 10 hybridizations $225
FP067 FISH CEP probe for human chromosome 14-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP071 FISH CEP probe for human chromosome 15-green Green 10 hybridizations $225
FP072 FISH CEP probe for human chromosome 15-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP076 FISH CEP probe for human chromosome 16-green Green 10 hybridizations $225
FP077 FISH CEP probe for human chromosome 16-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP081 FISH CEP probe for human chromosome 17-green Green 10 hybridizations $225
FP082 FISH CEP probe for human chromosome 17-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP086 FISH CEP probe for human chromosome 18-green Green 10 hybridizations $225
FP087 FISH CEP probe for human chromosome 18-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP091 FISH CEP probe for human chromosome 19-green Green 10 hybridizations $225
FP092 FISH CEP probe for human chromosome 19-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP096 FISH CEP probe for human chromosome 20-green Green 10 hybridizations $225
FP097 FISH CEP probe for human chromosome 20-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP101 FISH CEP probe for human chromosome 21-green Green 10 hybridizations $225
FP102 FISH CEP probe for human chromosome 21-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP106 FISH CEP probe for human chromosome 22-green Green 10 hybridizations $225
FP107 FISH CEP probe for human chromosome 22-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP111 FISH CEP probe for human chromosome X-green Green 10 hybridizations $225
FP112 FISH CEP probe for human chromosome X-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   
Click on the product description to view images of fluorescent signals resulting from hybridization of the selected VividFISH™ probe with mammalian chromosomes.

 

 

 

 

Buy Catalog # Description Color Quantity Price
FP116 FISH CEP probe for human chromosome Y-green Green 10 hybridizations $225
FP117 FISH CEP probe for human chromosome Y-orange Orange 10 hybridizations $225

 

 

   
Buy Catalog # Description Quantity Price User manual
FP204 VividFISH™ FFPE pretreatment kit 10 reactions $150 Download
   

   

 

Using FISH with CRISPR

Using VividFISH™ probes for genome editing applications

Immortalized mammalian cell lines, while providing convenient model systems for biomedical and pharmaceutical research, are often aneuploid. For example, the commonly-used human embryonic kidney cell line HEK293 is hypotriploid, with a modal chromosomal number of 64. Further, the ploidy of HEK293 and some other cell lines is not uniform among cells in a population. This deviation of ploidy from the normal diploid state in immortalized cell lines poses a challenge for genome editing applications, such as gene knockout and mutagenesis, which often require complete elimination of wild type gene function. Therefore, knowledge of the number of copies of a targeted gene to be edited could signicantly benefit a cell line-based genome editing project.

GeneCopoeia's VividFISH™ chromosome enumeration probes are powerful tools for determining target gene copy number in genome editing applications. Using VividFISH™ probes to determine target chromosome number in a cell line before undergoing a genome editing project can help guide clone screening for desired modifications. To illustrate this concept, GeneCopoeia used a VividFISH™ chromosome enumeration probe to determine chromosome copy number in the human synovial fibroblast cell line MH7A, in which the X chromosome HDAC6 gene was being knocked out using CRISPR. Using VivdFISH™, we determined that MH7A cells carry five copies of the X chromosome (Figure 1A). This information helped us identify single clones that contained all five copies of the HDAC6 gene carrying CRISPR-mediated frameshift mutations (Figure1B).

 

A  
 
 
B  
 

 

Figure 1. Using VividFISH™ chromosome enumeration probes to identify pentalleleic gene knockout in a mammalian cell line. A. Fluorescence in situ hybridization (FISH) analysis reveals the presence of 5 copies of the HDAC6 gene in MH7A cells. Unmodified MH7A cells (top) and the diploid human cell line HT1080 (bottom) were hybridized with VividFISH™ probes recognizing the X chromosome (green), and the Y chromosome (orange). Only the male cell line HT1080 hybridizes with the Y chromosome probe. Nuclei were stained with DAPI (blue). B. Sequence analysis from two single clones following transfection of MH7A cells with GeneCopoeia Genome-CRISPTM sgRNA-expressing plasmids targeting HDAC6 (NCBI geneID:10013). Each clone carries 5 distinct frame shift alleles generated in a single transfection.