GeneCopoeia’s GLuc-ON™ EGR1 transcriptional response element (TRE) lentiviral clone enables analysis of the effects on genes regulated by early growth respionse protein 1 (EGR1), such as  transforming growth factor-beta-1 (TGFB1) and fibronectin (FN1). EGR1 is a transcription factor that responds to mitogenic stimulation. EGR1 regulates many processes, such as neuron activity, wound healing, apopotosis, and angiogenesis, and is known to both suppress and promote tumor growth.

The GLuc-ON™ PKC EGR1 lentiviral clone contains tandem repeats of EGR1 upstream of a minimal promoter and secreted Gaussia luciferase reporter gene, which allows robust and sensitive analysis without lysing the cells.  The EGR1 response elements are engineered to provide strong activation with low background (Figure 1). The GLuc-ON™ EGR1 TRE lentiviral clone is provided as transfection-ready DNA. A negative (non-activating) control lentiviral clone is also available for purchase.