Cell-Based Validation Services

- For miRNA target, shRNA, promoter, or ORF clones

GeneCopoeia provides cell-based validation services to help researchers to validate the function and performance of expression clones using standard cell lines or customer’s own cell lines. The services cover ORF cDNA clone expression validation, shRNA knockdown validation, miRNA target validation and promoter activity validation.

To request a quote, please fill out the form below.

* Required fields

1. Contact information
Name :
Institution :
Institution e-mail :
   Please check if you Do Not wish to receive promotion and new product information
Telephone :
2. Your gene symbol: *
3. The specific accession number for your gene: *
4. What type of validation do you need? *

miRNA target validation
shRNA knockdown
Promoter activity
ORF expression validation
Others, please specify ( Please specify )

 

4.1.Please write down the specific miRNA(s) you'd like to validate for this gene's 3'UTR target *

5.What construct(s) would you like to use for this service? *
A construct from GeneCopoeia™
My own construct (You will need to provide us the construct with its full sequence data and vector map)

 

5.1.What type of vector do you need for your GeneCopoeia construct? *
non-viral vector
lentiviral vector
*For miRNA target validation, lentiviral vector is not available.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *

3′UTR miRNA Target Clones

Order Vector ID Reporter gene Tracking gene

pEZX-MT05

Gaussia luciferase (GLuc)

Secreted alkaline phosphatase (SEAP)

pEZX-MT01

Firefly luciferase (FLuc)

Renilla luciferase

I don’t have any preference

I need help to choose the right vector

This is the default vector.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *
Order Vector ID

Promoter

Reporter gene

Selection marker

psi-H1

H1

eGFP

Puromycin

psi-U6

U6

eGFP

Puromycin

psi-mH1

H1

mCherry

Puromycin

psi-mU6

U6

mCherry

Puromycin

psi-nH1

H1

N/A

Puromycin

psi-nU6

U6

N/A

Puromycin

I don’t have any preference

I need help to choose the right vector

This is the default vector.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *
Order Vector ID

Tracking gene

Reporter gene

Selection marker

pEZX-PG0

Secreted alkaline phosphatase (SEAP)

Gaussia luciferase (GLuc)

Puromycin

pEZX-PG02

N/A

Gaussia luciferase (GLuc)

Puromycin

pEZX-PF02

N/A

eGFP

Puromycin

pEZX-PM02

N/A

mCherry

Puromycin

I don’t have any preference

I need help to choose the right vector

This is the default vector.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *
Order Vector ID

Promoter

Reporter gene

Selection marker

psiHIV-H1

H1

eGFP

Puromycin

psiHIV-U6

U6

eGFP

Puromycin

psiHIV-mH1

H1

mCherry

Puromycin

psiHIV-mU6

U6

mCherry

Puromycin

psiHIV-nH1

H1

N/A

Puromycin

psiHIV-nU6

U6

N/A

Puromycin

I don’t have any preference

I need help to choose the right vector

This is the default vector.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *
Order Vector ID

Tracking gene

Reporter gene

Selection marker

pEZX-LvPG04

Secreted alkaline phosphatase (SEAP)

Gaussia luciferase (GLuc)

Puromycin

pEZX-LvPG02

N/A

Gaussia luciferase (GLuc)

Puromycin

pEZX-LvPF02

N/A

eGFP

Puromycin

pEZX-LvPM02

N/A

mCherry

Puromycin

I don’t have any preference

I need help to choose the right vector

This is the default vector.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *

OmicsLink™ Expression-Ready ORF cDNA Clones (Mammalian)

Order Vector ID

Promoter

Tag

Selection marker

pReceiver-M01

CMV

N-His

Neomycin

pReceiver-M51

CMV

C-His+IRES-eGFP

Neomycin

pReceiver-M77

CMV

C-His

Neomycin

pReceiver-M02

CMV

N/A

Neomycin

pReceiver-M67

CMV

N/A

Hygromycin

pReceiver-M68

CMV

N/A

Neomycin

pReceiver-M29

CMV

N-eGFP

Neomycin

pReceiver-M03

CMV

C-eGFP

Neomycin

pReceiver-M98

CMV

C-eGFP(monomeric)

Neomycin

pReceiver-M15

CMV

N-eYFP

Neomycin

pReceiver-M16

CMV

C-eYFP

Neomycin

pReceiver-M32

CMV

N-eCFP

Neomycin

pReceiver-M33

CMV

C-eCFP

Neomycin

pReceiver-M04

CMV

N-GST

Neomycin

pReceiver-M05

CMV

N-Avi

Neomycin

pReceiver-M48

CMV

N-Avi+IRES-Biotin ligase

Neomycin

pReceiver-M17

CMV

C-Avi

Neomycin

pReceiver-M62

CMV

C-Avi+IRES-Biotin ligase

Neomycin

pReceiver-M06

CMV

N-3xHA

Neomycin

pReceiver-M07

CMV

C-3xHA

Neomycin

pReceiver-M08

CMV

C-3xHA-His

Neomycin

pReceiver-M43

CMV

N-Myc

Neomycin

pReceiver-M45

CMV

C-3xHA+IRES-eGFP

Neomycin

pReceiver-M09

CMV

C-Myc

Neomycin

pReceiver-M10

CMV

C-Myc-His

Neomycin

pReceiver-M72

CMV

C-Myc+IRES2-eGFP

Neomycin

pReceiver-M11

CMV

N-Flag

Neomycin

pReceiver-M12

CMV

N-3XFlag

Neomycin

pReceiver-M13

CMV

C-Flag

Neomycin

pReceiver-M46

CMV

C-Flag+IRES-eGFP

Neomycin

pReceiver-M14

CMV

C-3XFlag

Neomycin

pReceiver-M49

CMV

N-HaloTag

Neomycin

pReceiver-M50

CMV

C-HaloTag

Neomycin

pReceiver-M55

CMV

N-mCherry

Neomycin

pReceiver-M56

CMV

C-mCherry

Neomycin

pReceiver-M61

CMV

IRES2-eGFP

Neomycin

I don’t have any preference

I need help to choose the right vector

to validate the protein expression for this clone, you need to provide the protein specific antibody.

 

5.2.Please select the vector backbone for your GeneCopoeia construct *

OmicsLink™ Expression-Ready ORF cDNA Clones (Mammalian)

Order Vector ID

Promoter

Tag

Selection marker

pReceiver-Lv105

CMV

N/A

Puromycin

pReceiver-Lv151

CMV

N/A

Neomycin

pReceiver-Lv152

CMV

N/A

Hygromycin

pReceiver-Lv153

CMV

N/A

N/A

pReceiver-Lv156

EF1a

N/A

Puromycin

pReceiver-Lv186

CMV

C-3xHA

N/A

pReceiver-Lv157

CMV

C-3xHA

Neomycin

pReceiver-Lv120

CMV

C-3xHA

Puromycin

pReceiver-Lv108

CMV

N-Avi

Puromycin

pReceiver-Lv124

CMV

C-Avi

Puromycin

pReceiver-Lv154

CMV

C-eCFP

Neomycin

pReceiver-Lv127

CMV

C-eCFP

Puromycin

pReceiver-Lv109

CMV

N-eCFP

Puromycin

pReceiver-Lv103

CMV

N-eGFP

Puromycin

pReceiver-Lv122

CMV

C-eGFP

Puromycin

pReceiver-Lv182

CMV

N-eGFP

Neomycin

pReceiver-Lv183

CMV

C-eGFP

Neomycin

pReceiver-Lv189

CMV

N-eGFP

N/A

pReceiver-Lv123

CMV

C-eYFP

Puromycin

pReceiver-Lv104

CMV

N-eYFP

Puromycin

pReceiver-Lv187

CMV

C-Flag

N/A

pReceiver-Lv101

CMV

N-Flag

Neomycin

pReceiver-Lv121

CMV

C-Flag

Puromycin

pReceiver-Lv102

CMV

N-Flag

Puromycin

pReceiver-Lv158

CMV

C-Flag

Neomycin

pReceiver-Lv203

CMV

C-Flag-SV40-eGFP-IRES-puromycin

Puromycin

pReceiver-Lv216

CMV

C-Flag-SV40-mCherry-IRES-puromycin

Puromycin

pReceiver-Lv129

CMV

C-HaloTag

Puromycin

pReceiver-Lv110

CMV

N-HaloTag

Puromycin

pReceiver-Lv211

CMV

C-HaloTag-IRES-hygromycin

Hygromycin

pReceiver-Lv128

CMV

C-His

Puromycin

pReceiver-Lv215

CMV

IRES2-eGFP

N/A

pReceiver-Lv205

CMV

IRES2-eGFP-IRES-puromycin

Puromycin

pReceiver-Lv220

CMV

IRES2-hygromycin

Hygromycin

pReceiver-Lv214

CMV

IRES2-mCherry

N/A

pReceiver-Lv213

CMV

IRES2-eGFP-IRES-puromycin

Puromycin

pReceiver-Lv111

CMV

N-mCherry

Puromycin

pReceiver-Lv130

CMV

C-mCherry

Puromycin

pReceiver-Lv155

CMV

C-mCherry

Neomycin

pReceiver-Lv107

CMV

N-Myc

Puromycin

pReceiver-Lv125

CMV

C-Myc

Puromycin

pReceiver-Lv126

CMV

C-Myc-His

Puromycin

pReceiver-Lv202

CMV

C-Myc-SV40-eGFP-IRES-puromycin

Puromycin

pReceiver-Lv204

CMV

C-Myc-SV40-mCherry-IRES-puromycin

Puromycin

pReceiver-Lv207

CMV

SV40-eGFP-IRES-hygromycin

Hygromycin

pReceiver-Lv201

CMV

SV40-eGFP-IRES-puromycin

Puromycin

pReceiver-Lv217

CMV

SV40-hLUC-IRES-puromycin

Puromycin

pReceiver-Lv206

CMV

SV40-mCherry-IRES-puromycin

Puromycin

pReceiver-Lv01

CMV

N/A

N/A

pReceiver-Lv36

CMV

IRES-luciferase

N/A

pReceiver-Lv76

CMV

IRES2-eGFP

N/A

pReceiver-Lv78

CMV

IRES2-eCFP

N/A

pReceiver-Lv79

CMV

IRES2-eYFP

N/A

pReceiver-Lv80

CMV

IRES2-mCherry

N/A

pReceiver-Lv81

CMV

IRES2-eGFP

N/A

pReceiver-Lv40

CMV

IRES2-Neomycin

Neomycin

pReceiver-Lv21

CMV

N/A

Neomycin

pReceiver-Lv02

CMV

C-3xHA

N/A

pReceiver-Lv52

CMV

C-3xHA+IRES2-eGFP

N/A

pReceiver-Lv06

CMV

C-3xHA

Neomycin

pReceiver-Lv23

CMV

N-Flag

Neomycin

pReceiver-Lv03

CMV

C-Flag

N/A

pReceiver-Lv53

CMV

C-Flag+IRES2-eGFP

N/A

pReceiver-Lv07

CMV

C-Flag

Neomycin

pReceiver-Lv19

CMV

N-eGFP

Neomycin

pReceiver-Lv04

CMV

C-eGFP

N/A

pReceiver-Lv08

CMV

C-eGFP

Neomycin

pReceiver-Lv20

CMV

N-eYFP

Neomycin

pReceiver-Lv05

CMV

C-eYFP

N/A

pReceiver-Lv09

CMV

C-eYFP

Neomycin

pReceiver-Lv34

CMV

N-eCFP

Neomycin

pReceiver-Lv61

CMV

C-eCFP

N/A

pReceiver-Lv62

CMV

C-eCFP

Neomycin

pReceiver-Lv35

CMV

N-Avi+IRES-Biotin ligase

N/A

pReceiver-Lv68

CMV

C-Avi+IRES-Biotin ligase

N/A

pReceiver-Lv26

CMV

N-Avi

Neomycin

pReceiver-Lv10

CMV

C-Avi

Neomycin

pReceiver-Lv25

CMV

N-Myc

Neomycin

pReceiver-Lv17

CMV

C-Myc

Neomycin

pReceiver-Lv18

CMV

C-Myc-His

Neomycin

pReceiver-Lv45

CMV

C-Myc+IRES-eCFP

N/A

pReceiver-Lv46

CMV

C-Myc+IRES-luciferase

N/A

pReceiver-Lv47

CMV

C-Myc+IRES-Neomycin

Neomycin

pReceiver-Lv48

CMV

C-Myc+IRES2-eYFP

N/A

pReceiver-Lv70

CMV

C-Myc+IRES2-eYFP

N/A

pReceiver-Lv75

CMV

C-Myc+IRES2-mCherry

N/A

pReceiver-Lv77

PKG

C-Myc+IRES2-eGFP

N/A

pReceiver-Lv64

CMV

N-HaloTag

Neomycin

pReceiver-Lv65

CMV

C-HaloTag

Neomycin

pReceiver-Lv71

CMV

N-mCherry

Puromycin

pReceiver-Lv72

CMV

C-mCherry

Neomycin

pReceiver-Lv73

CMV

C-His+IRES-eGFP

N/A

pReceiver-Lv41

EF1a

N/A

Neomycin

pReceiver-Lv66

CMV

N/A

Hygromycin

pReceiver-Lv67

CMV

N/A

Puromycin

I don’t have any preference

I need help to choose the right vector

to validate the protein expression for this clone, you need to provide the protein specific antibody.

6.Please indicate the species that you are interested in *
Human
Mouse
Rat
Other species ( Please indicate )
7. The cell line used for validation *
293T (human)
H1299 (human)
NIH3T3 (mouse)
I don’t have any preference
I’ll provide my own cell line

 

7.1. The name and culture condition of your specific cell line *

(You will need to provide us the cell line with its detailed culture condition and other references)

8. What would you like to do next after the construct is validated?
I would like to inquire about the lentiviral packaging service for this construct
I would like to inquire about the recombinant protein production service for this construct
I would like to inquire about the stable cell line development service for this construct
I would like to inquire about the lentiviral packaging services for this construct
I would like to inquire about the stable cell line service for the construct
*For miRNA target validation, these options are not available.
*For non-GeneCopoeia construct, these options are not available.

9.Any special requirements and comments, please enter them here:

10. Need more help? Please contact us at
Email: inquiry@genecopoeia.com
Call: 866-360-9531 or 301-762-0888
Otherwise, please click Next to view the summary of your selections