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miProfile™ Custom miRNA qPCR Arrays

For expression profiling of custom-specified miRNAs

miProfile™ Custom miRNA qPCR Arrays profile the expression of customer specified miRNAs. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of miRNAs and replicates to be analyzed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility:

  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.
  • Negative controls(NC): monitors the sample quality

For the best performance, All-in-One™ miRNA First-Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

To view other miProfile miRNA qPCR arrays, click below:

 

Key advantages

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Validated primers

  • Each primer is designed using a proprietary algorithm and has been experimentally  validated

Flexible array design

  • GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.

Robust performance

  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer has been experimental validated for specific amplification
  • Broad linearity – Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates

 

Cat. No. Product name Species Number of miRNAs Number of plates Document
PAM-CS96 miProfile™ miRNA qPCR arrays custom services N/A Variable Variable Variable

Select RT-PCR reagents

Buy Catalog# Product Description Price
QP002 All-in-One™ qPCR Mix (600 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $389
QP001 All-in-One™ qPCR Mix (200 qPCR reactions) High-fidelity, hot-start DNA polymerase,ROX reference dye,optimized reaction buffer and dNTPs $169
QP020 RNAzol® RT RNA Isolation Reagent Easy isolation of mRNA, microRNA or total RNA $69
QP018 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (60 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $515
QP005 All-in-One™ qPCR Mix (4000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $1995
QP017 All-in-One™ miRNA First-Strand cDNA Synthesis Kit for miRNA qPCR array (20 RT reactions) Poly A Polymerase, RTase Mix, PAP/RT buffer, spike-in control (for use with miRNA qPCR arrays) $225
QP004 All-in-One™ qPCR Mix (1000 qPCR reactions) High-fidelity, hot-start DNA polymerase, ROX reference dye, optimized reaction buffer and dNTPs $635

 

 

Data analysis tool

Analyze the qPCR result using GeneCopoeia's online Data Analysis System (free).

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