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Click Chemistry Tools

Introduction

Click Chemistry is a new approach to rapidly and selectively react ("click") a pair of functional groups with each other in mild, aqueous conditions. Click Chemistry reactions can be categorized into two separate groups: (1) Cu(I)-catalyzed Azide-Alkyne Click Chemistry reaction (CuAAC); (2) Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC).
Biomolecules that have been functionalized with a CLICK moiety (e.g. a terminal Alkyne or Azide) can be detected by labeling with a compatible CLICKable fluorescent dye (e.g. a fluorescent Azide or Alkyne) that allows the subsequent visualization by fluorescence spectroscopy. GeneCopoeia has developed a series of CLICKable biotin and fluorescent dyes for efficient “click” labeling of biomolecules.

Features

  • Highly selective, low background labeling
  • Rapid and quantitative labeling
  • Multiple fluorophore selection for multicolor imaging
  • Andy Fluor™ dye for brighter signal

 
Figure 1. Click chemistry labeling

Standard CuAAC reactions
Azides and alkynes can be used interchangeably in standard CuAAC reactions, so either one can serve as the tagged substrate or be labeled for the detection step. Some biomolecules are sensitive to the amount of copper used to catalyze standard click reactions. In particular, proteins such as GFP and R-PE exhibit reduced fluorescence and nucleic acids can be denatured by a high-copper environment.

Picolyl azides for low-copper reactions
Picolyl azide reagents enable researchers to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the original azide/alkyne click reaction. Picolyl azide only need much lower copper concentration than standard click reactions to protect against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin). Additionally the picolyl azides can be used to increase sensitivity or accelerate the reactions by using the original copper-catalyzed reaction conditions.

DBCO alkynes for copper-free reactions
DBCO reagents can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.

CLICKable Biotin and Fluorescent Dye Selection Guide

Label Alkyne Azide DBCO
Biotin C303 C304  C330
5-FAM C305 C306  
5-TAMRA C307 C308  
Texas Red C309 C310  
Cy3 C311 C312 C335
Cy5 C313 C314 C336
Andy Fluor™ 350 C315 C316  
Andy Fluor™ 488 C317 C318 C331
Andy Fluor™ 555 C319 C320 C332
Andy Fluor™ 594 C321 C322 C333
Andy Fluor™ 647 C323 C324 C334

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C303 Biotin Alkyne 5 mg $90
C304 Biotin Azide 5 mg $90
C305 5-FAM Alkyne 495/518 1 µmol $90
C306 5-FAM Azide 495/518 1 µmol $90
C307 5-TAMRA Alkyne 555/575 1 µmol $120
C308 5-TAMRA Azide 555/575 1 µmol $120
C309 Texas Red Alkyne 594/614 1 µmol $150
C310 Texas Red Azide 594/614 1 µmol $150
C311 Cy3 Alkyne 555/565 1 µmol $180
C312 Cy3 Azide 555/565 1 µmol $180
C313 Cy5 Alkyne 650/665 1 µmol $180
C314 Cy5 Azide 650/665 1 µmol $180
C315 Andy Fluor 350 Alkyne 350/440 1 µmol $250
C316 Andy Fluor 350 Azide 350/440 1 µmol $250
C317 Andy Fluor 488 Alkyne 505/526 1 µmol $250
C318 Andy Fluor 488 Azide 505/526 1 µmol $250
C319 Andy Fluor 555 Alkyne 553/565 1 µmol $250
C320 Andy Fluor 555 Azide 553/565 1 µmol $250
C321 Andy Fluor 594 Alkyne 590/615 1 µmol $250
C322 Andy Fluor 594 Azide 590/615 1 µmol $250
C323 Andy Fluor 647 Alkyne 650/666 1 µmol $250
C324 Andy Fluor 647 Azide 650/666 1 µmol $250
C330 Biotin DBCO 1 mg $180
C331 Andy Fluor 488 DBCO 495/520 0.5 µmol $250
C332 Andy Fluor 555 DBCO 553/565 0.5 µmol $250
C333 Andy Fluor 594 DBCO 590/615 0.5 µmol $250
C334 Andy Fluor 647 DBCO 650/665 0.5 µmol $250
C335 Cy3 DBCO 555/565 0.5 µmol $250
C336 Cy5 DBCO 650/665 0.5 µmol $250