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Cell Apoptosis Assays

Introduction

Apoptosis (programmed cell death) is the genetically controlled ablation of cells during normal development. Apoptosis is distinct from necrosis in both the biochemical and the morphological changes that occur. In contrast to necrotic cells, apoptotic cells are characterized morphologically by compaction of the nuclear chromatin, shrinkage of the cytoplasm and production of membrane-bound apoptotic bodies.

Biochemically, apoptosis is distinguished by fragmentation of the genome and cleavage or degradation of several cellular proteins. Several approaches have been developed to study apoptosis. Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach for accurate assessment (Figure 1).

GeneCopoeia offers a wide array of apoptosis assays for measuring multiple components on a choice of assay platforms:

  •  Annexin V binding assays
  •  Caspase activity assays
  •  Mitochondrial membrane potential assays
  •  DNA fragmentation and morphology
 

Annexin V Binding Assays
Our annexin V conjugates and kits provide quick and reliable detection methods for studying the externalization of phosphatidylserine in apoptotic cells.

 

Caspase Activity Assays
A distinctive feature of the early stage apoptosis is the activation of caspase enzymes. GeneCopoeia offers a wide array of caspase assays in live and dead cells.

 

Mitochondrial Membrane Potential Assays
A distinctive feature of the early stage apoptosis of programmed cell death is the disruption of active mitochondria. These changes can be monitored using our extensive selection of potential-sensitive mitochondrial stains.

 

DNA Fragmentation and Morphology
A distinctive feature of the late stage apoptosis is the DNA fragmentation and nuclear changes. Our TUNEL assays provide quick and reliable detection methods for studying in situ apoptotic DNA fragmentation.

Figure 1. Cell Apoptosis Pathway
Figure 2. Cell Apoptosis Detection Methods

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
A025 eGFP Annexin V and PI Apoptosis Kit 475/509 100 assays $180
A026 FITC Annexin V and PI Apoptosis Kit 490/519 100 assays $180
A027 Andy Fluor™ 488 Annexin V and PI Apoptosis Kit 495/520 100 assays $180
A028 Apoptotic, Necrotic, and Healthy Cells Detection Kit - 50 assays $150
A029A
A029B
Annexin V, recombinant -
0.5 mg
1.0 mg
$650
$1200
A030 eGFP Annexin V, 100 assays 475/509 25 μg $140
A031 Biotin-X Annexin V, 100 assays - 25 μg $140
A032 Andy Fluor™ 350 Annexin V, 100 assays 347/440 25 μg $140
A033 FITC Annexin V, 100 assays 490/519 25 μg $140
A034 Andy Fluor™ 488 Annexin V, 100 assays 495/520 25 μg $140
A035 Andy Fluor™ 555 Annexin V, 100 assays 553/565 25 μg $140
A036 Andy Fluor™ 568 Annexin V, 100 assays 578/602 25 μg $140
A037 Andy Fluor™ 594 Annexin V, 100 assays 590/615 25 μg $140
A038 Andy Fluor™ 647 Annexin V, 100 assays 650/665 25 μg $140
A039 Andy Fluor™ 680 Annexin V, 100 assays 680/700 25 μg $140
A041 5X Annexin V Binding Buffer - 50 mL $40
A042 Caspase-3/7 Substrate   Z-DEVD-R110 496/520 5 mg $400
A043A
A043B
Caspase-3/7 Inhibitor   Ac-DEVD-CHO -
1 mg
5 mg
$80
$250
A044 Caspase-3/7 Z-DEVD-R110 Assay Kit 496/520 100 assays $200
A045 Caspase-3/7 Z-DEVD-R110 HTS Assay Kit 496/520 100 assays $260
A046 LIVE Caspase-3/7 Green Detection Reagent 500/530 100 μL $180
A047 LIVE Caspase-3/7 Green Apoptosis Assay Kit 500/530 100 assays $200
A048 JC-1 Mitochondrial Membrane Potential Detection Kit 514/529, 590 100 assays $180
A049 TUNEL Chromogenic Apoptosis Detection Kit - 50 assays $380
A050 TUNEL Andy Fluor™ 488 Apoptosis Detection Kit 495/520 50 assays $380
A051 TUNEL Andy Fluor™ 594 Apoptosis Detection Kit 590/615 50 assays $380
A052 TUNEL Andy Fluor™ 647 Apoptosis Detection Kit 650/665 50 assays $380