You are here: Home > Product > Avitag >
| OmicsLink™ AviTag™ Human ORF Expression Clones |
Human AviTagged ORF clones are available in both T7 and CMV promoter driven expression systems. AviTag™ technology is based on biotinylation of AviTag by Biotin ligase in vitro or in vivo and on the specific and reverse binding of avidin or streptavidin to biotin for immobilizing, purifying and visualizing proteins.
|
Applications of AviTagged clones and fusion proteins: |
- Immobilized AviTag-fusion proteins are used for applications ranging from high-throughput screening (HTS) to protein-protein interactions using surface plasmon resonance.
- Purification of AviTag-fusion proteins using monomeric avidin can be more efficient than other commonly technologies such as hexa-HIS technology.
- AviTag-fusion proteins can be visualized using avidin or streptavidin conjugates such as Western Blots and MHC tetramers for staining and sorting T cells.
|
| Advantages of Biotin-AviTag system over chemical labeling of proteins with biotin: |
|
- Almost any protein can now be biotinylated either in vivo or in vitro easily and efficiently at this single and unique AviTag site.
- Because the biotinylation is performed enzymatically, the reaction conditions are very gentle and the labeling is highly specific.
- Biotin-AviTag has 15 amino acids, which is only one fifth of the bulk of alternative biotinylation tag sequences that are over 85 amino acid residues long; an important consideration if steric conflicts is to be minimized.
|
 |
>> ORF clones are also available in 80 or so other
expression vector systems |
| >> Applications and advantages of human ORF clones |
| AviTag™ Clones' related Products |
|
|
 |
| |
|
| AviTag™ is a trademark of Avidity, LLC. AviTag™ technology (U.S. Patent Nos. 5,723,584, 5,874,239 & 5,932,433) is owned by and licensed from Avidity, LLC, Denver, Colorado. |
|
