GeneCopoeia's Anti-Human proBDNF Antibody is a mouse monoclonal antibody that is specific for the prodomain of human brainderived neurotrophic factor (proBDNF). It does not recognize mature BDNF.
Purified GST-prodomain protein was used to immunize mice as well as to screen hybridoma clones. Clone mAb287
was selected from the initial screening and further characterized using recombinant proteins, neurons, brain lysates and
sections from bdnf KO and the wild type littermates as described1.
The anti-proBDNF antibody recognizes the prodomain of human BDNF and does not recognize mature BDNF
Western blot, immunoprecipitation, immunostaining and affinity purification.
Figure 1. Characterization of anti-prodomain antibody. (a) Western blot analysis documenting immunospecificity of monoclonal mAb287 antibody. Purified recombinant mature BDNF (50 ng) and purified recombinant proBDNF (50 ng) were separated using SDSPAGE and probed with anti-mature BDNF antibody (N-20, Santa Cruz) (left panel) or the monoclonal mAb287 antibody (right panel). (b) Detection of proBDNF from brain lysates using mAb287 antibody. The hippocampal lysates of bdnf -/-, bdnf +/+ and bdnf-HA/HA mice were immunoprecipitated with mAb287 antibody. After the separateion by SDS-PAGE, immunobloting was performed using either anti-mAb287, anti-HA.11 or anti-mature BDNF antibodies as indicated. Note the lack of cleaved prodomain, which has predicted mass of 14-16 kDa.