Vector-Based Human microRNA Expression Clones and Target Validation Expression Clones

Available Now:

• 650 pre-made OmicsLink™ human pre-miRNA expression clones
• miRNA target sequence expression clones that cover 20,000 human genes

OmicsLink™ Human Pre-miRNA Expression Clones

• 650 human precursor miRNA expression clones are available which covers more than 95% of 678 known miRNAs in release 11.0 in miRBase
• The expression cassettes of all miRNA expression clones have been fully sequenced
• Lentiviral vector based expression constructs allow efficient infection based transduction of miRNAs into non-dividing and difficult to transfect target cells as well as transfection based transduction into conventinal dividing cells
• Stable cell line selection marker (neomycin) enables studies of the effect of both long term and transient expression regulation
• Vector plasmid transfection and virus infection transduction efficiency are monitored by EGFP reporter protein.

• Full length 3' UTR expression constructs
• Firefly Luciferase reporter gene and assay for miRNA gene expression regulation
• Renilla Luciferase reporter gene and assay as internal control
  
  

Applications of Precursor miRNA and Target Expression Clones

• Gene expression regulation by miRNA.
• MiRNA target gene identification and validation.